- 36-3100 - Invitrogen Antibodies SDC4 antibody

Submitted references Altered shear stress on endothelial cells leads to remodeling of extracellular matrix and induction of angiogenesis.

Russo TA, Banuth AMM, Nader HB, Dreyfuss JL
PloS one 2020;15(11):e0241040

Syndecan-4 regulates extravillous trophoblast migration by coordinating protein kinase C activation.

Jeyarajah MJ, Jaju Bhattad G, Kops BF, Renaud SJ
Scientific reports 2019 Jul 15;9(1):10175

Syndecan-4 influences mammalian myoblast proliferation by modulating myostatin signalling and G1/S transition.

Keller-Pinter A, Szabo K, Kocsis T, Deak F, Ocsovszki I, Zvara A, Puskas L, Szilak L, Dux L
FEBS letters 2018 Sep;592(18):3139-3151

A targeted functional RNA interference screen uncovers glypican 5 as an entry factor for hepatitis B and D viruses.

Verrier ER, Colpitts CC, Bach C, Heydmann L, Weiss A, Renaud M, Durand SC, Habersetzer F, Durantel D, Abou-Jaoudé G, López Ledesma MM, Felmlee DJ, Soumillon M, Croonenborghs T, Pochet N, Nassal M, Schuster C, Brino L, Sureau C, Zeisel MB, Baumert TF
Hepatology (Baltimore, Md.) 2016 Jan;63(1):35-48

Syndecan-4 knockout leads to reduced extracellular transglutaminase-2 and protects against tubulointerstitial fibrosis.

Scarpellini A, Huang L, Burhan I, Schroeder N, Funck M, Johnson TS, Verderio EA
Journal of the American Society of Nephrology : JASN 2014 May;25(5):1013-27

Heparan sulfate proteoglycans are required for cellular binding of the hepatitis E virus ORF2 capsid protein and for viral infection.

Kalia M, Chandra V, Rahman SA, Sehgal D, Jameel S
Journal of virology 2009 Dec;83(24):12714-24

Hypoxia increases macrophage motility, possibly by decreasing the heparan sulfate proteoglycan biosynthesis.

Asplund A, Ostergren-Lundén G, Camejo G, Stillemark-Billton P, Bondjers G
Journal of leukocyte biology 2009 Aug;86(2):381-8

CCN2 (connective tissue growth factor) promotes fibroblast adhesion to fibronectin.

Chen Y, Abraham DJ, Shi-Wen X, Pearson JD, Black CM, Lyons KM, Leask A
Molecular biology of the cell 2004 Dec;15(12):5635-46

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of (A) T47D, (B) HepG2, (C) NCI-H69, and (D) JEG-3 cell lysates using Rb anti-Syndecan-4 (Product # 36-3100).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of (A) T47D, (B) HepG2, (C) NCI-H69, and (D) JEG-3 cell lysates using Rb anti-Syndecan-4 (Product # 36-3100).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Syndecan-4 showing staining in the cytoplasm of paraffin-embedded human colon tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Syndecan-4 polyclonal antibody (Product # 36-3100) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 4 Proteoglycans and connexin immunofluorescence and gene expression of ECs exposed to shear stress (4 dyn/cm 2 or 12 dyn/cm 2 ) or ECs in static conditions. (A) Location of syndecan-4 by immunofluorescence analyzed with confocal microscopy. (B) Analysis of the gene expression of syndecan-4 core protein by qPCR. (C) Location of perlecan by immunofluorescence analyzed with confocal microscopy. (D) Analysis of the gene expression of perlecan core protein by qPCR. (E) Location of Connexin 43 by immunofluorescence using confocal microscopy. (F) Analysis of the gene expression of Connexin 43 by qPCR. Red: Alexa Fluor 594 secondary antibody for staining of syndecan-4 or perlecan; Green: Alexa Fluor 488 secondary antibody for staining of Connexin 43; Blue: DAPI, nuclei staining. 630 X magnification. Scale bar = 50 mum. Sdc4: Syndecan-4. Con43: Connexin 43. RE: Relative expression to GAPDH. *p < 0.01; **p < 0.001; ***p < 0.0001 (ANOVA, Bonferroni test). The assay was performed in triplicate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 SDC4 expression in HTR8 EVTs and human placental tissue. RT-PCR analysis showing expression of SDC1 , SDC2 , SDC3 , SDC4 , and RNA18SN1 ( 18S ) in ( a ) HTR8 EVTs and ( b ) human placenta at 6, 11, 14, and 39 weeks of gestation. ( c ) Quantitative RT-PCR analysis of relative SDC4 expression in lysates of human placental tissue at 6, 11, 14, and 39 weeks of gestation. ( d ) Western blot showing SDC4 protein expression in lysates of human placental tissue at 6, 11, 14, and 39 weeks of gestation. Tubulin was used as a loading control. ( e ) Immunohistochemistry of SDC4 in paraffin-embedded sections of 6, 14, and 39-week human placenta. Human Leukocyte Antigen-G (HLA-G) and cytokeratin staining denotes EVTs and trophoblasts, respectively. Hematoxylin is used to highlight nuclei. Rabbit IgG control images are located in the bottom left corner of the SDC4 panels. ( f ) Quantitative RT-PCR and ( g ) western blot analysis of SDC4 transcript and protein expression in HTR8 EVTs exposed to ambient (Amb) and low O 2 conditions. Graphs represent means (SEM). Scale bar = 100 mum. Uncropped images of DNA gels and western blots are provided in Figs S4 and S5 , respectively. Values significantly different from controls are indicated with an asterisk (* P < 0.05; *** P < 0.001; **** P < 0.0001).

36-3100

Antibody data