HPA005716

antibody from Atlas Antibodies

Targeting: SDC4 amphiglycan, ryudocan, SYND4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA005716

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA005716, RRID:AB_1080160

Product name

Anti-SDC4

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

PQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGS
GDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQ
VPTEPKKLEENEVIPKRISPVEESEDVSNKVSMSS
TVQGSNIFERTEV


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and SDC4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401051).

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & the Golgi apparatus.

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

Sample type

HUMAN