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- Antibody Data
- Antigen structure
- References [24]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AF1556 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Podocalyxin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Podocalyxin and rhEndoglycan is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9R0M4- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Foxc2 is essential for podocyte function.
Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis.
Mice doubly deficient in Six4 and Six5 show ventral body wall defects reproducing human omphalocele.
Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury.
Loss of ezrin expression reduced the susceptibility to the glomerular injury in mice.
Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy.
aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability.
BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer.
Loss of mucin-type O-glycans impairs the integrity of the glomerular filtration barrier in the mouse kidney.
Deletion of inositol-requiring enzyme-1α in podocytes disrupts glomerular capillary integrity and autophagy.
Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1.
EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance.
The development and plasticity of alveolar type 1 cells.
Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice.
Endothelial ALK1 Is a Therapeutic Target to Block Metastatic Dissemination of Breast Cancer.
Direct action of endothelin-1 on podocytes promotes diabetic glomerulosclerosis.
Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling.
Clustered PI(4,5)P₂ accumulation and ezrin phosphorylation in response to CLIC5A.
Protein tyrosine phosphatase 1B inhibition protects against podocyte injury and proteinuria.
Deficiency for endoglin in tumor vasculature weakens the endothelial barrier to metastatic dissemination.
Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) λ/ι.
Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function.
Novel regulators of kidney development from the tips of the ureteric bud.
Nilsson D, Heglind M, Arani Z, Enerbäck S
Physiological reports 2019 May;7(9):e14083
Physiological reports 2019 May;7(9):e14083
Endophilin-A2 dependent VEGFR2 endocytosis promotes sprouting angiogenesis.
Genet G, Boyé K, Mathivet T, Ola R, Zhang F, Dubrac A, Li J, Genet N, Henrique Geraldo L, Benedetti L, Künzel S, Pibouin-Fragner L, Thomas JL, Eichmann A
Nature communications 2019 May 28;10(1):2350
Nature communications 2019 May 28;10(1):2350
Mice doubly deficient in Six4 and Six5 show ventral body wall defects reproducing human omphalocele.
Takahashi M, Tamura M, Sato S, Kawakami K
Disease models & mechanisms 2018 Oct 25;11(10)
Disease models & mechanisms 2018 Oct 25;11(10)
Reducing Pericyte-Derived Scarring Promotes Recovery after Spinal Cord Injury.
Dias DO, Kim H, Holl D, Werne Solnestam B, Lundeberg J, Carlén M, Göritz C, Frisén J
Cell 2018 Mar 22;173(1):153-165.e22
Cell 2018 Mar 22;173(1):153-165.e22
Loss of ezrin expression reduced the susceptibility to the glomerular injury in mice.
Hatano R, Takeda A, Abe Y, Kawaguchi K, Kazama I, Matsubara M, Asano S
Scientific reports 2018 Mar 14;8(1):4512
Scientific reports 2018 Mar 14;8(1):4512
Volumetric, Nanoscale Optical Imaging of Mouse and Human Kidney via Expansion Microscopy.
Chozinski TJ, Mao C, Halpern AR, Pippin JW, Shankland SJ, Alpers CE, Najafian B, Vaughan JC
Scientific reports 2018 Jul 10;8(1):10396
Scientific reports 2018 Jul 10;8(1):10396
aPKC controls endothelial growth by modulating c-Myc via FoxO1 DNA-binding ability.
Riddell M, Nakayama A, Hikita T, Mirzapourshafiyi F, Kawamura T, Pasha A, Li M, Masuzawa M, Looso M, Steinbacher T, Ebnet K, Potente M, Hirose T, Ohno S, Fleming I, Gattenlöhner S, Aung PP, Phung T, Yamasaki O, Yanagi T, Umemura H, Nakayama M
Nature communications 2018 Dec 17;9(1):5357
Nature communications 2018 Dec 17;9(1):5357
BMP9, but not BMP10, acts as a quiescence factor on tumor growth, vessel normalization and metastasis in a mouse model of breast cancer.
Ouarné M, Bouvard C, Boneva G, Mallet C, Ribeiro J, Desroches-Castan A, Soleilhac E, Tillet E, Peyruchaud O, Bailly S
Journal of experimental & clinical cancer research : CR 2018 Aug 30;37(1):209
Journal of experimental & clinical cancer research : CR 2018 Aug 30;37(1):209
Loss of mucin-type O-glycans impairs the integrity of the glomerular filtration barrier in the mouse kidney.
Song K, Fu J, Song J, Herzog BH, Bergstrom K, Kondo Y, McDaniel JM, McGee S, Silasi-Mansat R, Lupu F, Chen H, Bagavant H, Xia L
The Journal of biological chemistry 2017 Oct 6;292(40):16491-16497
The Journal of biological chemistry 2017 Oct 6;292(40):16491-16497
Deletion of inositol-requiring enzyme-1α in podocytes disrupts glomerular capillary integrity and autophagy.
Kaufman DR, Papillon J, Larose L, Iwawaki T, Cybulsky AV
Molecular biology of the cell 2017 Jun 15;28(12):1636-1651
Molecular biology of the cell 2017 Jun 15;28(12):1636-1651
Efficient activation of the lymphangiogenic growth factor VEGF-C requires the C-terminal domain of VEGF-C and the N-terminal domain of CCBE1.
Jha SK, Rauniyar K, Karpanen T, Leppänen VM, Brouillard P, Vikkula M, Alitalo K, Jeltsch M
Scientific reports 2017 Jul 7;7(1):4916
Scientific reports 2017 Jul 7;7(1):4916
EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance.
Depner C, Zum Buttel H, Böğürcü N, Cuesta AM, Aburto MR, Seidel S, Finkelmeier F, Foss F, Hofmann J, Kaulich K, Barbus S, Segarra M, Reifenberger G, Garvalov BK, Acker T, Acker-Palmer A
Nature communications 2016 Jul 29;7:12329
Nature communications 2016 Jul 29;7:12329
The development and plasticity of alveolar type 1 cells.
Yang J, Hernandez BJ, Martinez Alanis D, Narvaez del Pilar O, Vila-Ellis L, Akiyama H, Evans SE, Ostrin EJ, Chen J
Development (Cambridge, England) 2016 Jan 1;143(1):54-65
Development (Cambridge, England) 2016 Jan 1;143(1):54-65
Simultaneous targeted activation of Notch1 and Vhl-disruption in the kidney proximal epithelial tubular cells in mice.
Johansson E, Rönö B, Johansson M, Lindgren D, Möller C, Axelson H, Smith EM
Scientific reports 2016 Aug 5;6:30739
Scientific reports 2016 Aug 5;6:30739
Endothelial ALK1 Is a Therapeutic Target to Block Metastatic Dissemination of Breast Cancer.
Cunha SI, Bocci M, Lövrot J, Eleftheriou N, Roswall P, Cordero E, Lindström L, Bartoschek M, Haller BK, Pearsall RS, Mulivor AW, Kumar R, Larsson C, Bergh J, Pietras K
Cancer research 2015 Jun 15;75(12):2445-56
Cancer research 2015 Jun 15;75(12):2445-56
Direct action of endothelin-1 on podocytes promotes diabetic glomerulosclerosis.
Lenoir O, Milon M, Virsolvy A, Hénique C, Schmitt A, Massé JM, Kotelevtsev Y, Yanagisawa M, Webb DJ, Richard S, Tharaux PL
Journal of the American Society of Nephrology : JASN 2014 May;25(5):1050-62
Journal of the American Society of Nephrology : JASN 2014 May;25(5):1050-62
Endothelial Notch activity promotes angiogenesis and osteogenesis in bone.
Ramasamy SK, Kusumbe AP, Wang L, Adams RH
Nature 2014 Mar 20;507(7492):376-380
Nature 2014 Mar 20;507(7492):376-380
Crim1 maintains retinal vascular stability during development by regulating endothelial cell Vegfa autocrine signaling.
Fan J, Ponferrada VG, Sato T, Vemaraju S, Fruttiger M, Gerhardt H, Ferrara N, Lang RA
Development (Cambridge, England) 2014 Jan;141(2):448-59
Development (Cambridge, England) 2014 Jan;141(2):448-59
Clustered PI(4,5)P₂ accumulation and ezrin phosphorylation in response to CLIC5A.
Al-Momany A, Li L, Alexander RT, Ballermann BJ
Journal of cell science 2014 Dec 15;127(Pt 24):5164-78
Journal of cell science 2014 Dec 15;127(Pt 24):5164-78
Protein tyrosine phosphatase 1B inhibition protects against podocyte injury and proteinuria.
Kumagai T, Baldwin C, Aoudjit L, Nezvitsky L, Robins R, Jiang R, Takano T
The American journal of pathology 2014 Aug;184(8):2211-24
The American journal of pathology 2014 Aug;184(8):2211-24
Deficiency for endoglin in tumor vasculature weakens the endothelial barrier to metastatic dissemination.
Anderberg C, Cunha SI, Zhai Z, Cortez E, Pardali E, Johnson JR, Franco M, Páez-Ribes M, Cordiner R, Fuxe J, Johansson BR, Goumans MJ, Casanovas O, ten Dijke P, Arthur HM, Pietras K
The Journal of experimental medicine 2013 Mar 11;210(3):563-79
The Journal of experimental medicine 2013 Mar 11;210(3):563-79
Def-6, a novel regulator of small GTPases in podocytes, acts downstream of atypical protein kinase C (aPKC) λ/ι.
Worthmann K, Leitges M, Teng B, Sestu M, Tossidou I, Samson T, Haller H, Huber TB, Schiffer M
The American journal of pathology 2013 Dec;183(6):1945-1959
The American journal of pathology 2013 Dec;183(6):1945-1959
Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function.
Pierchala BA, Muñoz MR, Tsui CC
Kidney international 2010 Nov;78(9):868-82
Kidney international 2010 Nov;78(9):868-82
Novel regulators of kidney development from the tips of the ureteric bud.
Schmidt-Ott KM, Yang J, Chen X, Wang H, Paragas N, Mori K, Li JY, Lu B, Costantini F, Schiffer M, Bottinger E, Barasch J
Journal of the American Society of Nephrology : JASN 2005 Jul;16(7):1993-2002
Journal of the American Society of Nephrology : JASN 2005 Jul;16(7):1993-2002
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Podocalyxin by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Podocalyxin in Mouse Thymus. Podocalyxin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1556) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling when primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to high endothelial venules. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
