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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Immunocytochemistry [1]
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- Product number
- MAB1556 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Podocalyxin Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Podocalyxin in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q9R0M4- Isotype
- IgG
- Antibody clone number
- 192703
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Sequential formation and resolution of multiple rosettes drive embryo remodelling after implantation.
Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.
Modulation of apical constriction by Wnt signaling is required for lung epithelial shape transition.
In vivo RNA interference models of inducible and reversible Sirt1 knockdown in kidney cells.
Podocalyxin selectively marks erythroid-committed progenitors during anemic stress but is dispensable for efficient recovery.
A new mouse model of immune-mediated podocyte injury.
Christodoulou N, Kyprianou C, Weberling A, Wang R, Cui G, Peng G, Jing N, Zernicka-Goetz M
Nature cell biology 2018 Nov;20(11):1278-1289
Nature cell biology 2018 Nov;20(11):1278-1289
Repression of Interstitial Identity in Nephron Progenitor Cells by Pax2 Establishes the Nephron-Interstitium Boundary during Kidney Development.
Naiman N, Fujioka K, Fujino M, Valerius MT, Potter SS, McMahon AP, Kobayashi A
Developmental cell 2017 May 22;41(4):349-365.e3
Developmental cell 2017 May 22;41(4):349-365.e3
Modulation of apical constriction by Wnt signaling is required for lung epithelial shape transition.
Fumoto K, Takigawa-Imamura H, Sumiyama K, Kaneiwa T, Kikuchi A
Development (Cambridge, England) 2017 Jan 1;144(1):151-162
Development (Cambridge, England) 2017 Jan 1;144(1):151-162
In vivo RNA interference models of inducible and reversible Sirt1 knockdown in kidney cells.
Chuang PY, Xu J, Dai Y, Jia F, Mallipattu SK, Yacoub R, Gu L, Premsrirut PK, He JC
The American journal of pathology 2014 Jul;184(7):1940-56
The American journal of pathology 2014 Jul;184(7):1940-56
Podocalyxin selectively marks erythroid-committed progenitors during anemic stress but is dispensable for efficient recovery.
Maltby S, Hughes MR, Zbytnuik L, Paulson RF, McNagny KM
Experimental hematology 2009 Jan;37(1):10-8
Experimental hematology 2009 Jan;37(1):10-8
A new mouse model of immune-mediated podocyte injury.
Meyer TN, Schwesinger C, Wahlefeld J, Dehde S, Kerjaschki D, Becker JU, Stahl RA, Thaiss F
Kidney international 2007 Oct;72(7):841-52
Kidney international 2007 Oct;72(7):841-52
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Podocalyxin in bEnd.3 Mouse Cell Line. Podocalyxin was detected in immersion fixed bEnd.3 mouse endothelioma cell line using 10 µg/mL Rat Anti-Mouse Podocalyxin Monoclonal Antibody (Catalog # MAB1556) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
