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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- V2227 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Podocalyxin Antibody
- Antibody type
- Monoclonal
- Antigen
- A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Rat, Rabbit
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 3D3
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Elevated expression of podocalyxin is associated with lymphatic invasion, basal-like phenotype, and clinical outcome in axillary lymph node-negative breast cancer.
The anti-adhesive mucin podocalyxin may help initiate the transperitoneal metastasis of high grade serous ovarian carcinoma.
Release of podocalyxin into the extracellular space. Role of metalloproteinases.
Impaired transcription factor interplay in addition to advanced glycation end products suppress podocalyxin expression in high glucose-treated human podocytes.
The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma.
Enhanced podocalyxin expression alters the structure of podocyte basal surface.
Overexpression of the anti-adhesin podocalyxin is an independent predictor of breast cancer progression.
Puromycin aminonucleoside suppresses integrin expression in cultured glomerular epithelial cells.
Identification of podocalyxin-like protein as a high endothelial venule ligand for L-selectin: parallels to CD34.
Forse CL, Yilmaz YE, Pinnaduwage D, O'Malley FP, Mulligan AM, Bull SB, Andrulis IL
Breast cancer research and treatment 2013 Feb;137(3):709-19
Breast cancer research and treatment 2013 Feb;137(3):709-19
The anti-adhesive mucin podocalyxin may help initiate the transperitoneal metastasis of high grade serous ovarian carcinoma.
Cipollone JA, Graves ML, Köbel M, Kalloger SE, Poon T, Gilks CB, McNagny KM, Roskelley CD
Clinical & experimental metastasis 2012 Mar;29(3):239-52
Clinical & experimental metastasis 2012 Mar;29(3):239-52
Release of podocalyxin into the extracellular space. Role of metalloproteinases.
Fernández D, Larrucea S, Nowakowski A, Pericacho M, Parrilla R, Ayuso MS
Biochimica et biophysica acta 2011 Aug;1813(8):1504-10
Biochimica et biophysica acta 2011 Aug;1813(8):1504-10
Impaired transcription factor interplay in addition to advanced glycation end products suppress podocalyxin expression in high glucose-treated human podocytes.
Drossopoulou GI, Tsotakos NE, Tsilibary EC
American journal of physiology. Renal physiology 2009 Sep;297(3):F594-603
American journal of physiology. Renal physiology 2009 Sep;297(3):F594-603
The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma.
Schopperle WM, DeWolf WC
Stem cells (Dayton, Ohio) 2007 Mar;25(3):723-30
Stem cells (Dayton, Ohio) 2007 Mar;25(3):723-30
Enhanced podocalyxin expression alters the structure of podocyte basal surface.
Economou CG, Kitsiou PV, Tzinia AK, Panagopoulou E, Marinos E, Kershaw DB, Kerjaschki D, Tsilibary EC
Journal of cell science 2004 Jul 1;117(Pt 15):3281-94
Journal of cell science 2004 Jul 1;117(Pt 15):3281-94
Overexpression of the anti-adhesin podocalyxin is an independent predictor of breast cancer progression.
Somasiri A, Nielsen JS, Makretsov N, McCoy ML, Prentice L, Gilks CB, Chia SK, Gelmon KA, Kershaw DB, Huntsman DG, McNagny KM, Roskelley CD
Cancer research 2004 Aug 1;64(15):5068-73
Cancer research 2004 Aug 1;64(15):5068-73
Puromycin aminonucleoside suppresses integrin expression in cultured glomerular epithelial cells.
Krishnamurti U, Zhou B, Fan WW, Tsilibary E, Wayner E, Kim Y, Kashtan CE, Michael A
Journal of the American Society of Nephrology : JASN 2001 Apr;12(4):758-66
Journal of the American Society of Nephrology : JASN 2001 Apr;12(4):758-66
Identification of podocalyxin-like protein as a high endothelial venule ligand for L-selectin: parallels to CD34.
Sassetti C, Tangemann K, Singer MS, Kershaw DB, Rosen SD
The Journal of experimental medicine 1998 Jun 15;187(12):1965-75
The Journal of experimental medicine 1998 Jun 15;187(12):1965-75
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human samples
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of angiosarcoma stained with Podocalyxin antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human placenta with Podocalyxin antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS surface staining of NCCIT cells with Podocalyxin antibody
