330602

antibody from BioLegend

Targeting: PODXL Gp200, PC, PCLP

Western blot

Immunocytochemistry

Immunoprecipitation

Radioimmunoassay

Antibody data

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Validation data

Product number: 330602 - Provider product page
Provider: BioLegend
Proper citation: BioLegend Cat#330602, RRID:AB_1186144
Product name: Purified anti-human TRA-1-60-R
Antibody type: Monoclonal
Antigen: Human embryonal carinoma cell line 2102Ep cl.2A6
Description: TRA-1-60-R antibody recognizes the neuraminidase resistant form of TRA-1-60. TRA-1-60 is a 200-240 kD cell surface antigen expressed by human embryonic stem cells (ESC), embryonic germ cells (EG), and embryonal carcinoma cells. The expression of TRA-1-60 on human ESC is downregulated upon differentiation. The soluble form of TRA-1-60 has been found in the serum of patients with embryonic cancers. Historically, TRA-1-60 is a neuroaminidase-sensitive (TRA-1-60-S) carbohydrate epitope expressed on podocalyxin, a member of the mucin-like glycoprotein CD34 family. Podocalyxin is a type I transmembrane sialoprotein with anti-adhesion properties. Further studies have shown that some of TRA-1-60 is neuraminidase resistant (TRA-1-60-R). The two forms of TRA-1-60 are essentially indistinguishable in terms of their cellular reactivity except for the difference in neuraminidase sensitivity. Both of TRA-1-60-S and TRA-1-60-R appear to be the same molecular weight glycoproteins detected by Western blot.
Reactivity: Human
Host: Mouse
Conjugate: Unconjugated
Isotype: IgM
Vial size: 100 µg
Concentration: 0.5 mg/ml
Storage: The antibody solution should be stored undiluted at 4°C.

PODXL protein structure - 330602 shown in red.