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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NB300-597 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-597, RRID:AB_10001236
- Product name
- Rabbit Polyclonal Adenosine A2aR Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects adenosine receptor A2a. This does not detect other AR subtypes.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 uL
- Concentration
- 0.69 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references CD73 is associated with poor prognosis in HNSCC.
Ren ZH, Lin CZ, Cao W, Yang R, Lu W, Liu ZQ, Chen YM, Yang X, Tian Z, Wang LZ, Li J, Wang X, Chen WT, Ji T, Zhang CP
Oncotarget 2016 Sep 20;7(38):61690-61702
Oncotarget 2016 Sep 20;7(38):61690-61702
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Adenosine A2aR Antibody [NB300-597] - Simple Western lane view shows a specific band for Adenosine A2a R in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Adenosine A2aR Antibody [NB300-597] - Analysis of 25 ug of human placenta (lane 1), HepG2 (lane 2), Hela (lane 3) and mouse liver (lane 4) cell lysates.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NB300-597] - Analysis showing positive staining in the cytoplasm and membrane of Human placenta tissue (right) compared with a negative control in the absence of primary antibody (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NB300-597] - Analysis showing positive staining in the cytoplasm and membrane of Human testis tissue (right) compared with a negative control in the absence of primary antibody (left).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Adenosine A2aR Antibody [NB300-597] - Staining in the cytoplasm and membrane of Mouse testis tissue (right) compared with a negative control in the absence of primary antibody (left).
