- GTX101669 - GeneTex ACTN4 antibody

Submitted references UBL3 modification influences protein sorting to small extracellular vesicles.

Ageta H, Ageta-Ishihara N, Hitachi K, Karayel O, Onouchi T, Yamaguchi H, Kahyo T, Hatanaka K, Ikegami K, Yoshioka Y, Nakamura K, Kosaka N, Nakatani M, Uezumi A, Ide T, Tsutsumi Y, Sugimura H, Kinoshita M, Ochiya T, Mann M, Setou M, Tsuchida K
Nature communications 2018 Sep 26;9(1):3936

Analysis of the thrombotic and fibrinolytic activities of tumor cell-derived extracellular vesicles.

Durrieu L, Bharadwaj A, Waisman DM
Blood advances 2018 May 22;2(10):1054-1065

Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes.

Kowal J, Arras G, Colombo M, Jouve M, Morath JP, Primdal-Bengtson B, Dingli F, Loew D, Tkach M, Théry C
Proceedings of the National Academy of Sciences of the United States of America 2016 Feb 23;113(8):E968-77

Remodeling the zonula adherens in response to tension and the role of afadin in this response.

Choi W, Acharya BR, Peyret G, Fardin MA, Mège RM, Ladoux B, Yap AS, Fanning AS, Peifer M
The Journal of cell biology 2016 Apr 25;213(2):243-60

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extract D. 30 ?g HepG2 whole cell lysate/extract E. 30 ?g A375 whole cell lysate/extract7.5% SDS-PAGEalpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extract B. 30 ?g GL261 whole cell lysate/extract C. 30 ?g C8D30 whole cell lysate/extract D. 30 ?g NIH-3T3 whole cell lysate/extract E. 30 ?g BCL-1 whole cell lysate/extractF. 30 ?g Raw264.7 whole cell lysate/extract G. 30 ?g C2C12 whole cell lysate/extract7.5% SDS-PAGEalpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (50 ?g of whole cell lysate) A: Mouse brain 5% SDS PAGE GTX101669 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract7.5% SDS-PAGEalpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using alpha Actinin 4(GTX101669) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded A549 xenograft, using alpha Actinin 4(GTX101669) antibody at 1:500 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:500.

GTX101669

Antibody data