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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA1-26928 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Sarcomeric alpha Actin Monoclonal Antibody (5C5)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- This antibody is specific to alpha-skeletal and alpha-cardiac muscle actins. It does not react with smooth muscle tissue.
- Antibody clone number
- 5C5
Submitted references NF-κB inhibitor on Toll-like receptor 4 signal-induced expression of angiotensinogen and AT1a receptor in neonatal rat left ventricular myocytes.
Valvular cardiac myxoma in a dog.
ALK-ATIC fusion in urinary bladder inflammatory myofibroblastic tumor.
Jiang H, Wang HY, Wang JW, Lou DY, Niu N, Li GH, Qu P
Experimental and therapeutic medicine 2018 Nov;16(5):3875-3882
Experimental and therapeutic medicine 2018 Nov;16(5):3875-3882
Valvular cardiac myxoma in a dog.
Akkoc A, Ozyigit MO, Cangul IT
Journal of veterinary medicine. A, Physiology, pathology, clinical medicine 2007 Sep;54(7):356-8
Journal of veterinary medicine. A, Physiology, pathology, clinical medicine 2007 Sep;54(7):356-8
ALK-ATIC fusion in urinary bladder inflammatory myofibroblastic tumor.
Debiec-Rychter M, Marynen P, Hagemeijer A, Pauwels P
Genes, chromosomes & cancer 2003 Oct;38(2):187-90
Genes, chromosomes & cancer 2003 Oct;38(2):187-90
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Sarcomeric alpha actin (green) in cultured primary cardiomyocytes. Primary cardiomyocytes were isolated and cultured using the Primary Cardiomyocyte Isolation Kit (Product # 88281). Cells were grown in 24-well plates (seeded at 5 x 105 cells/well) or in 35mm glass bottom plates (at a density of 2.5 x 106 cells/well). At day 1 and day 7, cells were fixed with 4% paraformaldehyde, permeablilized with 0.1% Triton X-100 in HBSS for 10 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were probed with a Sarcomeric alpha actin monoclonal antibody (Product # MA1-26928) at dilution of 1:300 for 2 hours at room temperature or overnight at 4 °C, washed with HBSS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at dilution of 1:500 for 1 hour at room temperature. Nuclei (blue) were visualized using Hoechst 33342 (Product # 62249). Images were taken at 20X magnification on a Carl Zeiss microscope (AxioVision Rel. 4.7).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical (Paraffin) analysis of human skeletal muscle tissue using (Product # MA1-26928) Sarcomeric alpha Actin Monoclonal Antibody (5C5).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1. Immunocytochemistry detection of striated muscle-specific sarcomeric alpha-actin x200. Positive staining of alpha-actin related antigen is a brown particle in the cytoplasm.
