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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA1-16906 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nkx3.1 Monoclonal Antibody (0361)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Suggested positive control: antigen standard for NKX3 (transient overexpression lysate), LNCAP cell extracts, mouse testis protein, mouse testis tissue lysate.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 0361
- Vial size
- 100 µL
- Concentration
- 2.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Inhibitor of differentiation 4 (ID4) inactivation promotes de novo steroidogenesis and castration-resistant prostate cancer.
Patel D, Knowell AE, Korang-Yeboah M, Sharma P, Joshi J, Glymph S, Chinaranagari S, Nagappan P, Palaniappan R, Bowen NJ, Chaudhary J
Molecular endocrinology (Baltimore, Md.) 2014 Aug;28(8):1239-53
Molecular endocrinology (Baltimore, Md.) 2014 Aug;28(8):1239-53
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of NKX3.1 in mouse testis lysate using MA1-16906. ECL detection 1 minute.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Nkx3.1 in mouse testis lysate. Samples were incubated in Nkx3.1 monoclonal antibody (Product # MA1-16906). ECL detection 1 minute.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Nkx3.1 in immersion fixed paraffin-embedded sections of mouse testis. Samples were incubated in Nkx3.1 monoclonal antibody (Product # MA1-16906) using a dilution of 1:300 for 1 hour at room temperature followed by the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nuclei in sperm cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
