11649-2-AP

antibody from Invitrogen Antibodies

Targeting: EIF1AX eIF-1A, eIF-4C, EIF1A, EIF4C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

11649-2-AP

Provider

Invitrogen Antibodies

Product name

EIF1AX Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MPKNKGKGG KNRRRGKNEN ESEKRELVFK EDGQEYAQVI KMLGNGRLEA MCFDGVKRLC HIRGKLRKKV WINTSDIILV GLRDYQDNKA DVILKYNADE ARSLKAYGEL PEHAKINETD TFGPGDDDEI QFDDIGDDDE DIDDI (1-144 aa encoded by BC000793)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.15 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11649-2-AP (EIF1AX antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 11649-2-AP (EIF1AX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 11649-2-AP (EIF1AX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas using 11649-2-AP (EIF1AX antibody) at dilution of 1:50 (under 10x lens).