A01752-1

antibody from Boster Biological Technology

Targeting: SFRP2 FRP-2, SARP1, SDF-5

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

A01752-1

Provider

Boster Biological Technology

Product name

Anti-SFRP2 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for SFRP2 detection. Tested with WB, ICC/IF, FCM in Human.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Flow Cytometry analysis of CACO-2 cells using anti-SFRP2 antibody (A01752-1). Overlay histogram showing CACO-2 cells stained with A01752-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SFRP2 Antibody (A01752-1, 1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Additional image