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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 66328-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SFRP2 Monoclonal Antibody (1F3A1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MLQGPGSLL LLFLASHCCL GSARGLFLFG QPDFSYKRSN CKPIPVNLQL CHGIEYQNMR LPNLLGHETM KEVLEQAGAW IPLVMKQCHP DTKKFLCSLF APVCLDDLDE TIQPCHSLCV QVKDRCAPVM SAFGFPWPDM LECDRFPQDN DLCIPLASSD HLLPATEEAP KVCEACKNKN DDDNDIMETL CKNDFALKIK VKEITYINRD TKIILETKSK TIYKLNGVSE RDLKKSVLWL KDSLQCTCEE MNDINAPYLV MGQKQGGELV ITSVKRWQKG QREFKRISRS IRKLQC (1-295 aa encoded by B C008666)
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1F3A1
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Y79 cells were subjected to SDS PAGE followed by western blot with 66328-1-Ig (SFRP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 66328-1-IG ( SFRP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed SH-SY5Y cells using 66328-1-IG (SFRP2 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human malignant melanoma tissue slide using 66328-1-IG ( SFRP2 Antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human malignant melanoma tissue slide using 66328-1-IG ( SFRP2 Antibody) at dilution of 1:200 (under 40x lens).
