Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-75673 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Secretin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references QiangGuYin Modulates the OPG/RANKL/RANK Pathway by Increasing Secretin Levels during Treatment of Primary Type I Osteoporosis.
Li X, Cui L, Chen W, Fang Y, Shen G, Li Z, Zhang B, Wu L
Evidence-based complementary and alternative medicine : eCAM 2021;2021:7114139
Evidence-based complementary and alternative medicine : eCAM 2021;2021:7114139
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Secretin in Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: H9C2 whole cell lysate. Samples were incubated with Secretin polyclonal antibody (Product # PA5-75673) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Secretin in Lane 1: HEK293T whole cell lysate, Lane 2: Raw264.7 whole cell lysate, Lane 3: H9C2 whole cell lysate. Samples were incubated with Secretin polyclonal antibody (Product # PA5-75673) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Effect of QiangGuYin (QGY) treatment on the protein expression of secretin, OPG, RANK, and RANKL in the femur tissue. (a-e) The protein expression of secretin and OPG was decreased, whereas that of RANK and RANKL was increased, after secretin knockdown. The expression of secretin and OPG was increased, whereas that of RANK and RANKL was significantly decreased in the Sec-kno + Q group, as compared to that in the Sec-kno group. Data are expressed as the mean +- SD ( n = 3). * P < 0.05 vs. the Con group; # P < 0.05 vs. the Sec-kno group. Groups were as follows: control group (Con), secretin overexpression group (Sec-over), secretin overexpression control group (Sec-over-Con), secretin overexpression + QGY group (Sec-over + Q), secretin knockdown group (Sec-kno), secretin knockdown control group (Sec-kno-Con), and secretin knockdown + QGY group (Sec-kno + Q).