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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00005274-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005274-M01, RRID:AB_425595
- Product name
- SERPINI1 monoclonal antibody (M01), clone 1D10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant SERPINI1.
- Antigen sequence
TGATFPEEAIADLSVNMYNRLRATGEDENILFSPL
SIALAMGMMELGAQGSTQEEIRHSMGYDSLKNGEE
FSFLKEFSNMVTAKESQYVMKIANSLFVQNGFHVN
EEFLQMMKKYFNAAVNHVDFSQNVAVANYINKWVE
NNTNNLVKDLVSPRDFDAATYLALINAVYFKGNWK
SQFRPENTRTFSFTKDDESEVQIPMMYQQGEFYYG
EFSDGSNEAGGIYQVLEIPYEGDEISMMLVLSRQE
VPLATLEPLVKAQLVEEWANSVKKQKVEVYLPRFT
VEQEIDLKDVLKALGITEIFIKDANLTGLYDNKEI
FLSKAIHKSFLEVNEEGSEAAAVSGMIAISRMAVL
YPQVIVDHPFFFLIRNRRTGTILFMGRVMHPETMN
TSGHDFEEL- Isotype
- IgG
- Antibody clone number
- 1D10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Proteomic analysis of podocyte exosome-enriched fraction from normal human urine.
Prunotto M, Farina A, Lane L, Pernin A, Schifferli J, Hochstrasser DF, Lescuyer P, Moll S
Journal of proteomics 2013 Apr 26;82:193-229
Journal of proteomics 2013 Apr 26;82:193-229
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- SERPINI1 monoclonal antibody (M01), clone 1D10 Western Blot analysis of SERPINI1 expression in HeLa ( Cat # L013V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged SERPINI1 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to SERPINI1 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
