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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-8023 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-8023, RRID:AB_627775
- Product name
- Anti-IFI16
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references IFI16 induction by glucose restriction in human fibroblasts contributes to autophagy through activation of the ATM/AMPK/p53 pathway.
Interferon-inducible IFI16, a negative regulator of cell growth, down-regulates expression of human telomerase reverse transcriptase (hTERT) gene.
AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC.
AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA.
Nucleolin is involved in interferon regulatory factor-2-dependent transcriptional activation
Duan X, Ponomareva L, Veeranki S, Choubey D
PloS one 2011 May 5;6(5):e19532
PloS one 2011 May 5;6(5):e19532
Interferon-inducible IFI16, a negative regulator of cell growth, down-regulates expression of human telomerase reverse transcriptase (hTERT) gene.
Song LL, Ponomareva L, Shen H, Duan X, Alimirah F, Choubey D
PloS one 2010 Jan 5;5(1):e8569
PloS one 2010 Jan 5;5(1):e8569
AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC.
Hornung V, Ablasser A, Charrel-Dennis M, Bauernfeind F, Horvath G, Caffrey DR, Latz E, Fitzgerald KA
Nature 2009 Mar 26;458(7237):514-8
Nature 2009 Mar 26;458(7237):514-8
AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA.
Fernandes-Alnemri T, Yu JW, Datta P, Wu J, Alnemri ES
Nature 2009 Mar 26;458(7237):509-13
Nature 2009 Mar 26;458(7237):509-13
Nucleolin is involved in interferon regulatory factor-2-dependent transcriptional activation
A Masumi, H Fukazawa, T Shimazu, M Yoshida, K Ozato, K Komuro, K Yamaguchi
Oncogene 2006 Apr;25(37):5113-5124
Oncogene 2006 Apr;25(37):5113-5124
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 83
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
