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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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- Product number
- PA5-51690 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFI16 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KEKFTPKKII AIANYVCRNG FLEVYPFTLV ADVNADRNME IPKGLIRSAS VTPKINQLCS QTKGSFVNGV FEVHKKNVRG EFTYYEIQDN TGKMEVVVHG RLTTINCEEG DKLKLTCFEL APKSGNTGEL RSVIHSHIKV IK
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IFI16 in control (vector only transfected HEK293T lysate) and IFI16 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a IFI16 Polyclonal Antibody (Product # PA5-51690).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IFI16 in human cell lines SK-MEL-30 and Caco-2 using a IFI16 Polyclonal Antibody (Product # PA5-51690). Corresponding IFI16 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-IFI16 Polyclonal Antibody (Product # PA5-51690) and bands at 82, 88 kDa corresponding to IFI16 were observed across the cell lines tested, except HeLa in which the protein expression was detected upon IFN gamma treatment. Modified whole cell extracts (1% SDS) (30ug lysate) of HeLa (Lane 1), HeLa treated with IFN gamma (15ng/ml for 16hr) (Lane 2), Raji (Lane 3), Ramos (Lane 4), Jurkat (Lane 5) and Daudi (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of IFI16 in human cell line U-251 MG shows positivity in nucleus & nucleoli. Samples were probed using an IFI16 Polyclonal Antibody (Product # PA5-51690).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of IFI16 in human cell line U-251 MG using an IFI16 Polyclonal Antibody (Product # PA5-51690) shows localization to nucleus and nucleoli.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IFI16 in human lymph node using IFI16 Polyclonal Antibody (Product # PA5-51690) shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of IFI16 in human skeletal muscle using IFI16 Polyclonal Antibody (Product # PA5-51690) shows low expression as expected.
