H00002167-M01

antibody from Abnova Corporation

Targeting: FABP4 A-FABP, aP2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00002167-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00002167-M01, RRID:AB_425423

Product name

FABP4 monoclonal antibody (M01), clone 2H3-1G10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant FABP4.

Antigen sequence

MCDAFVGTWKLVSSENFDDYMKEVGVGFATRKVAG
MAKPNMIISVNGDVITIKSESTFKNTEISFILGQE
FDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTI
KRKREDDKLVVECVMKGVTSTRVYERA


Isotype

IgG

Antibody clone number

2H3-1G10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

FABP4 monoclonal antibody (M01), clone 2H3-1G10. Western Blot analysis of FABP4 expression in human pancreas.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of FABP4 expression in transfected 293T cell line by FABP4 monoclonal antibody (M01), clone 2H3-1G10.Lane 1: FABP4 transfected lysate (Predicted MW: 14.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged FABP4 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of FABP4 transfected lysate using anti-FABP4 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with FABP4 MaxPab rabbit polyclonal antibody.

Validation comment

Immunoprecipitation

Protocol

Protocol