PA5-87384

antibody from Invitrogen Antibodies

Targeting: FABP4 A-FABP, aP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87384

Provider

Invitrogen Antibodies

Product name

FABP4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MCDAFVGTWK LVSSENFDDY MKEVGVGFAT RKVAGMAKPN MIISVNGDVI TIKSESTFKN TEISFILGQE FDEVTADDRK VKSTITLDGG VLVHVQKWDG KSTTIKRKRE DDKLVVECVM KGVTSTRVYE RA; Positive Samples: Mouse heart, Mouse trachea; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

2.4 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

FABP4 inhibitor BMS309403 protects against hypoxia-induced H9c2 cardiomyocyte apoptosis through attenuating endoplasmic reticulum stress.

Sun F, Du J, Li H, Hao S, Zhao G, Lu F
Journal of cellular and molecular medicine 2020 Oct;24(19):11188-11197

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using FABP4 Polyclonal antibody (Product # PA5-87384). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of FABP4 in extracts of various cell lines using FABP4 Polyclonal Antibody (Product # PA5-87384) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of FABP4 was performed in A549 cells using FABP4 Polyclonal Antibody (Product # PA5-87384).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of FABP4 in paraffin-embedded rat heart using FABP4 Polyclonal Antibody (Product # PA5-87384) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of FABP4 in paraffin-embedded human breast cancer using FABP4 Polyclonal Antibody (Product # PA5-87384) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of FABP4 in paraffin-embedded mouse heart using FABP4 Polyclonal Antibody (Product # PA5-87384) at a dilution of 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1 FIGURE FABP4 expression is induced in cardiomyocytes exposed to hypoxia. (A, B) H9c2 cardiomyocytes were subjected to hypoxia as indicated for increasing periods of time. Normoxia (0 h) is the control treatment. The mRNA level (A) and protein expression (B) of FABP4 in harvested H9c2 cardiomyocytes were assessed by the quantitative real-time PCR (qRT-PCR) analysis and Western blot analysis, respectively. beta-actin is the internal control for FABP4 expression. One-way analysis of variance (ANOVA) (n = 3). **, P < 0.01. (C) H9c2 cardiomyocytes were cultured under normoxia condition (0 h) or subjected to hypoxia for 24 h. FABP4 expression was visualized by the immunofluorescence method using the fluorescent-labelled antibody. The nuclei were stained with DAPI. Scale bar, 100 um. The intensity of FABP4 fluorescence was quantified by ImageJ. Student's t test (n = 5). **, P < 0.01