R34901

antibody from NSJ Bioreagents

Targeting: THBS1 THBS, THBS-1, TSP, TSP-1, TSP1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

R34901

Provider

NSJ Bioreagents

Product name

Thrombospondin Antibody

Antibody type

Polyclonal

Antigen

Amino acids NRIPESGGDNSVFD were used as the immunogen for this Thrombospondin antibody.

Description

Antigen affinity purified antibody

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

Aliquot and store the Thrombospondin antibody at -20°C.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse kidney lysate with Thrombospondin antibody at 1ug/ml. Expected molecular weight ~130 kDa (unmodified), 155~200 kDa (glycosylated).

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunoflorescence testing of permeabilized human HeLa cells with Thrombospondin antibody at 10ug/ml. Blue = DAPI nuclear counterstain.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunoflorescence testing of permeabilized human HepG2 cells with Thrombospondin antibody at 10ug/ml. Blue = DAPI nuclear counterstain.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS testing of fixed and permeabilized human A431 cells with Thrombospondin antibody at 10ug/10^6 cells.