MA5-13385

antibody from Invitrogen Antibodies

Targeting: THBS1 THBS, THBS-1, TSP, TSP-1, TSP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-13385

Provider

Invitrogen Antibodies

Product name

Thrombospondin 1 Monoclonal Antibody (D4.6)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-13385 targets Thrombospondin in IM and WB applications and shows reactivity with Bovine, Canine, Equine, Human, mouse, Ovine, Porcine, and Rat samples.

Antibody clone number

D4.6

Concentration

0.2 mg/mL

References

Submitted references

Effect of LSKL peptide on thrombospondin 1-mediated transforming growth factor β signal activation and liver regeneration after hepatectomy in an experimental model.

Kuroki H, Hayashi H, Nakagawa S, Sakamoto K, Higashi T, Nitta H, Hashimoto D, Chikamoto A, Beppu T, Baba H
The British journal of surgery 2015 Jun;102(7):813-25

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Regulation of T-lymphocyte motility, adhesion and de-adhesion by a cell surface mechanism directed by low density lipoprotein receptor-related protein 1 and endogenous thrombospondin-1.

Talme T, Bergdahl E, Sundqvist KG
Immunology 2014 Jun;142(2):176-92

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Long-term gene therapy with thrombospondin 2 inhibits TGF-β activation, inflammation and angiogenesis in chronic allograft nephropathy.

Daniel C, Vogelbacher R, Stief A, Grigo C, Hugo C
PloS one 2013;8(12):e83846

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Inflammatory cytokine-specific alterations in retinal endothelial cell function.

Palenski TL, Sorenson CM, Sheibani N
Microvascular research 2013 Sep;89:57-69

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Cyp1b1 mediates periostin regulation of trabecular meshwork development by suppression of oxidative stress.

Zhao Y, Wang S, Sorenson CM, Teixeira L, Dubielzig RR, Peters DM, Conway SJ, Jefcoate CR, Sheibani N
Molecular and cellular biology 2013 Nov;33(21):4225-40

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HGF regulates the activation of TGF-β1 in rat hepatocytes and hepatic stellate cells.

Narmada BC, Chia SM, Tucker-Kellogg L, Yu H
Journal of cellular physiology 2013 Feb;228(2):393-401

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A cytokine-controlled mechanism for integrated regulation of T-lymphocyte motility, adhesion and activation.

Bergström SE, Bergdahl E, Sundqvist KG
Immunology 2013 Dec;140(4):441-55

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A fibronectin-independent mechanism of collagen fibrillogenesis in adult liver remodeling.

Moriya K, Bae E, Honda K, Sakai K, Sakaguchi T, Tsujimoto I, Kamisoyama H, Keene DR, Sasaki T, Sakai T
Gastroenterology 2011 May;140(5):1653-63

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Expression of ADAMTS1 in endothelial cells is induced by shear stress and suppressed in sprouting capillaries.

Hohberg M, Knöchel J, Hoffmann CJ, Chlench S, Wunderlich W, Alter A, Maroski J, Vorderwülbecke BJ, Da Silva-Azevedo L, Knudsen R, Lehmann R, Fiedorowicz K, Bongrazio M, Nitsche B, Hoepfner M, Styp-Rekowska B, Pries AR, Zakrzewicz A
Journal of cellular physiology 2011 Feb;226(2):350-61

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The myc-miR-17~92 axis blunts TGF{beta} signaling and production of multiple TGF{beta}-dependent antiangiogenic factors.

Dews M, Fox JL, Hultine S, Sundaram P, Wang W, Liu YY, Furth E, Enders GH, El-Deiry W, Schelter JM, Cleary MA, Thomas-Tikhonenko A
Cancer research 2010 Oct 15;70(20):8233-46

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Tasquinimod (ABR-215050), a quinoline-3-carboxamide anti-angiogenic agent, modulates the expression of thrombospondin-1 in human prostate tumors.

Olsson A, Björk A, Vallon-Christersson J, Isaacs JT, Leanderson T
Molecular cancer 2010 May 17;9:107

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Morphine modulation of thrombospondin levels in astrocytes and its implications for neurite outgrowth and synapse formation.

Ikeda H, Miyatake M, Koshikawa N, Ochiai K, Yamada K, Kiss A, Donlin MJ, Panneton WM, Churchill JD, Green M, Siddiqui AM, Leinweber AL, Crews NR, Ezerskiy LA, Rendell VR, Belcheva MM, Coscia CJ
The Journal of biological chemistry 2010 Dec 3;285(49):38415-27

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Regulation of thrombospondin-1 by natural and synthetic progestins in human breast cancer cells.

Hyder SM, Liang Y, Wu J, Welbern V
Endocrine-related cancer 2009 Sep;16(3):809-17

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Estrogen regulation of thrombospondin-1 in human breast cancer cells.

Hyder SM, Liang Y, Wu J
International journal of cancer 2009 Sep 1;125(5):1045-53

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Fibrinogen is required for maintenance of platelet intracellular and cell-surface P-selectin expression.

Yang H, Lang S, Zhai Z, Li L, Kahr WH, Chen P, Brkić J, Spring CM, Flick MJ, Degen JL, Freedman J, Ni H
Blood 2009 Jul 9;114(2):425-36

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Extracellular matrix retention of thrombospondin 1 is controlled by its conserved C-terminal region.

Adams JC, Bentley AA, Kvansakul M, Hatherley D, Hohenester E
Journal of cell science 2008 Mar 15;121(Pt 6):784-95

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Improved vascular engraftment and graft function after inhibition of the angiostatic factor thrombospondin-1 in mouse pancreatic islets.

Olerud J, Johansson M, Lawler J, Welsh N, Carlsson PO
Diabetes 2008 Jul;57(7):1870-7

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The aberrant methylation of TSP1 suppresses TGF-beta1 activation in colorectal cancer.

Rojas A, Meherem S, Kim YH, Washington MK, Willis JE, Markowitz SD, Grady WM
International journal of cancer 2008 Jul 1;123(1):14-21

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Meteorin regulates angiogenesis at the gliovascular interface.

Park JA, Lee HS, Ko KJ, Park SY, Kim JH, Choe G, Kweon HS, Song HS, Ahn JC, Yu YS, Kim KW
Glia 2008 Feb;56(3):247-58

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Cell type-specific post-transcriptional regulation of production of the potent antiangiogenic and proatherogenic protein thrombospondin-1 by high glucose.

Bhattacharyya S, Marinic TE, Krukovets I, Hoppe G, Stenina OI
The Journal of biological chemistry 2008 Feb 29;283(9):5699-707

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Dendritic cell type determines the mechanism of bystander suppression by adaptive T regulatory cells specific for the minor antigen HA-1.

Derks RA, Jankowska-Gan E, Xu Q, Burlingham WJ
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3443-51

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Dendritic cell type determines the mechanism of bystander suppression by adaptive T regulatory cells specific for the minor antigen HA-1.

Derks RA, Jankowska-Gan E, Xu Q, Burlingham WJ
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):3443-51

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Differential regulation of thrombospondin-1 expression and antiangiogenesis of ECV304 cells by trichostatin A and helixor A.

Hong S, Chang SY, Yeom DH, Kang JH, Hong KJ
Anti-cancer drugs 2007 Oct;18(9):1005-14

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LTBP-1 blockade in dioxin receptor-null mouse embryo fibroblasts decreases TGF-beta activity: Role of extracellular proteases plasmin and elastase.

Gomez-Duran A, Mulero-Navarro S, Chang X, Fernandez-Salguero PM
Journal of cellular biochemistry 2006 Feb 1;97(2):380-92

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Barx2 functions through distinct corepressor classes to regulate hair follicle remodeling.

Olson LE, Zhang J, Taylor H, Rose DW, Rosenfeld MG
Proceedings of the National Academy of Sciences of the United States of America 2005 Mar 8;102(10):3708-13

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Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch.

Cambier S, Gline S, Mu D, Collins R, Araya J, Dolganov G, Einheber S, Boudreau N, Nishimura SL
The American journal of pathology 2005 Jun;166(6):1883-94

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Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch.

Cambier S, Gline S, Mu D, Collins R, Araya J, Dolganov G, Einheber S, Boudreau N, Nishimura SL
The American journal of pathology 2005 Jun;166(6):1883-94

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Autocrine regulation of T cell motility by calreticulin-thrombospondin-1 interaction.

Li SS, Forslöw A, Sundqvist KG
Journal of immunology (Baltimore, Md. : 1950) 2005 Jan 15;174(2):654-61

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Requirement of VPS33B, a member of the Sec1/Munc18 protein family, in megakaryocyte and platelet alpha-granule biogenesis.

Lo B, Li L, Gissen P, Christensen H, McKiernan PJ, Ye C, Abdelhaleem M, Hayes JA, Williams MD, Chitayat D, Kahr WH
Blood 2005 Dec 15;106(13):4159-66

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Mechanism of CD47-induced alpha4beta1 integrin activation and adhesion in sickle reticulocytes.

Brittain JE, Han J, Ataga KI, Orringer EP, Parise LV
The Journal of biological chemistry 2004 Oct 8;279(41):42393-402

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Ras modulates Myc activity to repress thrombospondin-1 expression and increase tumor angiogenesis.

Watnick RS, Cheng YN, Rangarajan A, Ince TA, Weinberg RA
Cancer cell 2003 Mar;3(3):219-31

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Disruption of anterior segment development by TGF-beta1 overexpression in the eyes of transgenic mice.

Flügel-Koch C, Ohlmann A, Piatigorsky J, Tamm ER
Developmental dynamics : an official publication of the American Association of Anatomists 2002 Oct;225(2):111-25

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T lymphocyte expression of thrombospondin-1 and adhesion to extracellular matrix components.

Li SS, Ivanoff A, Bergström SE, Sandström A, Christensson B, van Nerven J, Holgersson J, Hauzenberger D, Arencibia I, Sundqvist KG
European journal of immunology 2002 Apr;32(4):1069-79

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Mechanism of interaction of thrombospondin with human endothelium and inhibition of sickle erythrocyte adhesion to human endothelial cells by heparin.

Gupta K, Gupta P, Solovey A, Hebbel RP
Biochimica et biophysica acta 1999 Jan 6;1453(1):63-73

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Mechanism of interaction of thrombospondin with human endothelium and inhibition of sickle erythrocyte adhesion to human endothelial cells by heparin.

Gupta K, Gupta P, Solovey A, Hebbel RP
Biochimica et biophysica acta 1999 Jan 6;1453(1):63-73

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Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?

Shi SR, Cote RJ, Hawes D, Thu S, Shi Y, Young LL, Taylor CR
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999 Apr;47(4):463-70

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Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?

Shi SR, Cote RJ, Hawes D, Thu S, Shi Y, Young LL, Taylor CR
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999 Apr;47(4):463-70

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of Thrombospondin using Thrombospondin Monoclonal Antibody (Product # MA5-13385) on HUVEC Cells.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Thrombospondin 1 was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Thrombospondin 1 (D4.6) Mouse Monoclonal Antibody (Product # MA5-13385) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 LSKL (leucine-serine-lysine-leucine) peptide successfully inhibits transforming growth factor (TGF) beta-Smad signal activation induced by partial hepatectomy. a Assessment of phosphorylated Smad2 (pSmad2) nuclear localization. Immunohistochemical staining for pSmad2 at 6 h in mouse liver from normal saline and LSKL peptide groups. b Effects of LSKL peptide on pSmad2 expression in the regenerating liver at 6 h. Western blot analysis of pSmad2 in mouse liver from sham-operated, normal saline and LSKL peptide groups. beta-Actin served as a loading control. c Densitometric analysis for western blots of pSmad2 in mouse liver for the three groups. d Real-time PCR analysis of relative expression of Myc mRNA in mouse liver for the three groups. c,d Values are mean(s.d.). * P < 0*010 (Student's t test). e Western blotting of thrombospondin (TSP) 1 protein at 6 h after hepatectomy. Expression of TSP-1 was similar in normal saline and LSKL peptide groups