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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- NBP1-52410 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-52410, RRID:AB_11019218
- Product name
- Goat Polyclonal Thrombospondin-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Human THBS1/ Thrombospondin.
- Reactivity
- Human, Bovine, Porcine, Simian
- Host
- Goat
- Antigen sequence
aa19-32- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Thrombospondin-1 Antibody [NBP1-52410] - (1ug/ml) staining of Mouse Kidney lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of anti-THBS1 / Thrombospondin antibody with human placenta at concentration 3.75 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of anti-THBS1 / Thrombospondin antibody with human spleen at concentration 3.75 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of anti-THBS1 / Thrombospondin antibody with Spleenocytes. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Thrombospondin-1 Antibody [NBP1-52410] - Analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
