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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [40]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA5-13377 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Thrombospondin 1 Monoclonal Antibody (A4.1)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-13377 targets Thrombospondin in FACS, IF, and WB applications and shows reactivity with Bovine, Chicken, Human, mouse, and Rat samples.
- Antibody clone number
- A4.1
- Concentration
- 0.2 mg/mL
Submitted references Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme.
Adenosine stimulates angiogenesis by up-regulating production of thrombospondin-1 by macrophages.
Mutations in FKBP14 cause a variant of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss.
Oxidative stress-induced attenuation of thrombospondin-1 expression in primary rat astrocytes.
Glucose downregulation of PKG-I protein mediates increased thrombospondin1-dependent TGF-{beta} activity in vascular smooth muscle cells.
Role of thrombospondin 1 in macrophage inflammation in dysferlin myopathy.
Thrombospondin-1 regulates the normal prostate in vivo through angiogenesis and TGF-beta activation.
Regulation of thrombospondin-1 by natural and synthetic progestins in human breast cancer cells.
Estrogen regulation of thrombospondin-1 in human breast cancer cells.
Beta1 integrin cytoplasmic variants differentially regulate expression of the antiangiogenic extracellular matrix protein thrombospondin 1.
Shotgun proteomics implicates extracellular matrix proteins and protease systems in neuronal development induced by astrocyte cholinergic stimulation.
Angiostatic factors normally restrict islet endothelial cell proliferation and migration: implications for islet transplantation.
Angiostatic factors normally restrict islet endothelial cell proliferation and migration: implications for islet transplantation.
CCAAT box is required for the induction of human thrombospondin-1 gene by trichostatin A.
Meteorin regulates angiogenesis at the gliovascular interface.
Collagen-binding domains of gelatinase A and thrombospondin-derived peptides impede endocytic clearance of active gelatinase A and promote HT1080 fibrosarcoma cell invasion.
A new insight into phagocytosis of apoptotic cells: proteolytic enzymes divert the recognition and clearance of polymorphonuclear leukocytes by macrophages.
PPARalpha deficiency in inflammatory cells suppresses tumor growth.
A model of human tumor dormancy: an angiogenic switch from the nonangiogenic phenotype.
NOL7 is a nucleolar candidate tumor suppressor gene in cervical cancer that modulates the angiogenic phenotype.
Induction of TSP1 gene expression by heat shock is mediated via an increase in mRNA stability.
LTBP-1 blockade in dioxin receptor-null mouse embryo fibroblasts decreases TGF-beta activity: Role of extracellular proteases plasmin and elastase.
Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch.
Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch.
Trans-arachidonic acids generated during nitrative stress induce a thrombospondin-1-dependent microvascular degeneration.
Low density lipoprotein receptor-related protein mediates endocytic clearance of pro-MMP-2.TIMP-2 complex through a thrombospondin-independent mechanism.
Inhibiting cell proliferation during formation of the glial scar: effects on axon regeneration in the CNS.
Thrombospondin 1, a mediator of the antiangiogenic effects of low-dose metronomic chemotherapy.
Effects of cerivastatin on human arterial smooth muscle cell growth and extracellular matrix expression at varying glucose and low-density lipoprotein levels.
Antiangiogenic treatment with thrombospondin-1 enhances primary tumor radiation response and prevents growth of dormant pulmonary micrometastases after curative radiation therapy in human melanoma xenografts.
Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling.
Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling.
Thrombospondin-1 promotes fibroblast-mediated collagen gel contraction caused by activation of latent transforming growth factor beta-1.
Adhesion of normal erythrocytes at depressed venous shear rates to activated neutrophils, activated platelets, and fibrin polymerized from plasma.
Id1 regulates angiogenesis through transcriptional repression of thrombospondin-1.
Thrombospondin-1-mediated metastasis suppression by the primary tumor in human melanoma xenografts.
Mechanism of interaction of thrombospondin with human endothelium and inhibition of sickle erythrocyte adhesion to human endothelial cells by heparin.
Mechanism of interaction of thrombospondin with human endothelium and inhibition of sickle erythrocyte adhesion to human endothelial cells by heparin.
Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?
Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?
Jacquemin G, Wurmser A, Huyghe M, Sun W, Homayed Z, Merle C, Perkins M, Qasrawi F, Richon S, Dingli F, Arras G, Loew D, Vignjevic D, Pannequin J, Fre S
eLife 2022 May 11;11
eLife 2022 May 11;11
Adenosine stimulates angiogenesis by up-regulating production of thrombospondin-1 by macrophages.
Ernens I, Bousquenaud M, Lenoir B, Devaux Y, Wagner DR
Journal of leukocyte biology 2015 Jan;97(1):9-18
Journal of leukocyte biology 2015 Jan;97(1):9-18
Mutations in FKBP14 cause a variant of Ehlers-Danlos syndrome with progressive kyphoscoliosis, myopathy, and hearing loss.
Baumann M, Giunta C, Krabichler B, Rüschendorf F, Zoppi N, Colombi M, Bittner RE, Quijano-Roy S, Muntoni F, Cirak S, Schreiber G, Zou Y, Hu Y, Romero NB, Carlier RY, Amberger A, Deutschmann A, Straub V, Rohrbach M, Steinmann B, Rostásy K, Karall D, Bönnemann CG, Zschocke J, Fauth C
American journal of human genetics 2012 Feb 10;90(2):201-16
American journal of human genetics 2012 Feb 10;90(2):201-16
Oxidative stress-induced attenuation of thrombospondin-1 expression in primary rat astrocytes.
Chen JK, Zhan YJ, Yang CS, Tzeng SF
Journal of cellular biochemistry 2011 Jan;112(1):59-70
Journal of cellular biochemistry 2011 Jan;112(1):59-70
Glucose downregulation of PKG-I protein mediates increased thrombospondin1-dependent TGF-{beta} activity in vascular smooth muscle cells.
Wang S, Lincoln TM, Murphy-Ullrich JE
American journal of physiology. Cell physiology 2010 May;298(5):C1188-97
American journal of physiology. Cell physiology 2010 May;298(5):C1188-97
Role of thrombospondin 1 in macrophage inflammation in dysferlin myopathy.
De Luna N, Gallardo E, Sonnet C, Chazaud B, Dominguez-Perles R, Suarez-Calvet X, Gherardi RK, Illa I
Journal of neuropathology and experimental neurology 2010 Jun;69(6):643-53
Journal of neuropathology and experimental neurology 2010 Jun;69(6):643-53
Thrombospondin-1 regulates the normal prostate in vivo through angiogenesis and TGF-beta activation.
Fitchev PP, Wcislak SM, Lee C, Bergh A, Brendler CB, Stellmach VM, Crawford SE, Mavroudis CD, Cornwell ML, Doll JA
Laboratory investigation; a journal of technical methods and pathology 2010 Jul;90(7):1078-90
Laboratory investigation; a journal of technical methods and pathology 2010 Jul;90(7):1078-90
Regulation of thrombospondin-1 by natural and synthetic progestins in human breast cancer cells.
Hyder SM, Liang Y, Wu J, Welbern V
Endocrine-related cancer 2009 Sep;16(3):809-17
Endocrine-related cancer 2009 Sep;16(3):809-17
Estrogen regulation of thrombospondin-1 in human breast cancer cells.
Hyder SM, Liang Y, Wu J
International journal of cancer 2009 Sep 1;125(5):1045-53
International journal of cancer 2009 Sep 1;125(5):1045-53
Beta1 integrin cytoplasmic variants differentially regulate expression of the antiangiogenic extracellular matrix protein thrombospondin 1.
Goel HL, Moro L, Murphy-Ullrich JE, Hsieh CC, Wu CL, Jiang Z, Languino LR
Cancer research 2009 Jul 1;69(13):5374-82
Cancer research 2009 Jul 1;69(13):5374-82
Shotgun proteomics implicates extracellular matrix proteins and protease systems in neuronal development induced by astrocyte cholinergic stimulation.
Moore NH, Costa LG, Shaffer SA, Goodlett DR, Guizzetti M
Journal of neurochemistry 2009 Feb;108(4):891-908
Journal of neurochemistry 2009 Feb;108(4):891-908
Angiostatic factors normally restrict islet endothelial cell proliferation and migration: implications for islet transplantation.
Johansson A, Olerud J, Johansson M, Carlsson PO
Transplant international : official journal of the European Society for Organ Transplantation 2009 Dec;22(12):1182-8
Transplant international : official journal of the European Society for Organ Transplantation 2009 Dec;22(12):1182-8
Angiostatic factors normally restrict islet endothelial cell proliferation and migration: implications for islet transplantation.
Johansson A, Olerud J, Johansson M, Carlsson PO
Transplant international : official journal of the European Society for Organ Transplantation 2009 Dec;22(12):1182-8
Transplant international : official journal of the European Society for Organ Transplantation 2009 Dec;22(12):1182-8
CCAAT box is required for the induction of human thrombospondin-1 gene by trichostatin A.
Kang JH, Kim MJ, Chang SY, Sim SS, Kim MS, Jo YH
Journal of cellular biochemistry 2008 Jul 1;104(4):1192-203
Journal of cellular biochemistry 2008 Jul 1;104(4):1192-203
Meteorin regulates angiogenesis at the gliovascular interface.
Park JA, Lee HS, Ko KJ, Park SY, Kim JH, Choe G, Kweon HS, Song HS, Ahn JC, Yu YS, Kim KW
Glia 2008 Feb;56(3):247-58
Glia 2008 Feb;56(3):247-58
Collagen-binding domains of gelatinase A and thrombospondin-derived peptides impede endocytic clearance of active gelatinase A and promote HT1080 fibrosarcoma cell invasion.
Robinet A, Emonard H, Banyai L, Laronze JY, Patthy L, Hornebeck W, Bellon G
Life sciences 2008 Feb 13;82(7-8):376-82
Life sciences 2008 Feb 13;82(7-8):376-82
A new insight into phagocytosis of apoptotic cells: proteolytic enzymes divert the recognition and clearance of polymorphonuclear leukocytes by macrophages.
Guzik K, Bzowska M, Smagur J, Krupa O, Sieprawska M, Travis J, Potempa J
Cell death and differentiation 2007 Jan;14(1):171-82
Cell death and differentiation 2007 Jan;14(1):171-82
PPARalpha deficiency in inflammatory cells suppresses tumor growth.
Kaipainen A, Kieran MW, Huang S, Butterfield C, Bielenberg D, Mostoslavsky G, Mulligan R, Folkman J, Panigrahy D
PloS one 2007 Feb 28;2(2):e260
PloS one 2007 Feb 28;2(2):e260
A model of human tumor dormancy: an angiogenic switch from the nonangiogenic phenotype.
Naumov GN, Bender E, Zurakowski D, Kang SY, Sampson D, Flynn E, Watnick RS, Straume O, Akslen LA, Folkman J, Almog N
Journal of the National Cancer Institute 2006 Mar 1;98(5):316-25
Journal of the National Cancer Institute 2006 Mar 1;98(5):316-25
NOL7 is a nucleolar candidate tumor suppressor gene in cervical cancer that modulates the angiogenic phenotype.
Hasina R, Pontier AL, Fekete MJ, Martin LE, Qi XM, Brigaudeau C, Pramanik R, Cline EI, Coignet LJ, Lingen MW
Oncogene 2006 Jan 26;25(4):588-98
Oncogene 2006 Jan 26;25(4):588-98
Induction of TSP1 gene expression by heat shock is mediated via an increase in mRNA stability.
Kang JH, Kim SA, Hong KJ
FEBS letters 2006 Jan 23;580(2):510-6
FEBS letters 2006 Jan 23;580(2):510-6
LTBP-1 blockade in dioxin receptor-null mouse embryo fibroblasts decreases TGF-beta activity: Role of extracellular proteases plasmin and elastase.
Gomez-Duran A, Mulero-Navarro S, Chang X, Fernandez-Salguero PM
Journal of cellular biochemistry 2006 Feb 1;97(2):380-92
Journal of cellular biochemistry 2006 Feb 1;97(2):380-92
Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch.
Cambier S, Gline S, Mu D, Collins R, Araya J, Dolganov G, Einheber S, Boudreau N, Nishimura SL
The American journal of pathology 2005 Jun;166(6):1883-94
The American journal of pathology 2005 Jun;166(6):1883-94
Integrin alpha(v)beta8-mediated activation of transforming growth factor-beta by perivascular astrocytes: an angiogenic control switch.
Cambier S, Gline S, Mu D, Collins R, Araya J, Dolganov G, Einheber S, Boudreau N, Nishimura SL
The American journal of pathology 2005 Jun;166(6):1883-94
The American journal of pathology 2005 Jun;166(6):1883-94
Trans-arachidonic acids generated during nitrative stress induce a thrombospondin-1-dependent microvascular degeneration.
Kermorvant-Duchemin E, Sennlaub F, Sirinyan M, Brault S, Andelfinger G, Kooli A, Germain S, Ong H, d'Orleans-Juste P, Gobeil F Jr, Zhu T, Boisvert C, Hardy P, Jain K, Falck JR, Balazy M, Chemtob S
Nature medicine 2005 Dec;11(12):1339-45
Nature medicine 2005 Dec;11(12):1339-45
Low density lipoprotein receptor-related protein mediates endocytic clearance of pro-MMP-2.TIMP-2 complex through a thrombospondin-independent mechanism.
Emonard H, Bellon G, Troeberg L, Berton A, Robinet A, Henriet P, Marbaix E, Kirkegaard K, Patthy L, Eeckhout Y, Nagase H, Hornebeck W, Courtoy PJ
The Journal of biological chemistry 2004 Dec 24;279(52):54944-51
The Journal of biological chemistry 2004 Dec 24;279(52):54944-51
Inhibiting cell proliferation during formation of the glial scar: effects on axon regeneration in the CNS.
Rhodes KE, Moon LD, Fawcett JW
Neuroscience 2003;120(1):41-56
Neuroscience 2003;120(1):41-56
Thrombospondin 1, a mediator of the antiangiogenic effects of low-dose metronomic chemotherapy.
Bocci G, Francia G, Man S, Lawler J, Kerbel RS
Proceedings of the National Academy of Sciences of the United States of America 2003 Oct 28;100(22):12917-22
Proceedings of the National Academy of Sciences of the United States of America 2003 Oct 28;100(22):12917-22
Effects of cerivastatin on human arterial smooth muscle cell growth and extracellular matrix expression at varying glucose and low-density lipoprotein levels.
Siegel-Axel DI, Runge H, Seipel L, Riessen R
Journal of cardiovascular pharmacology 2003 Mar;41(3):422-33
Journal of cardiovascular pharmacology 2003 Mar;41(3):422-33
Antiangiogenic treatment with thrombospondin-1 enhances primary tumor radiation response and prevents growth of dormant pulmonary micrometastases after curative radiation therapy in human melanoma xenografts.
Rofstad EK, Henriksen K, Galappathi K, Mathiesen B
Cancer research 2003 Jul 15;63(14):4055-61
Cancer research 2003 Jul 15;63(14):4055-61
Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling.
Lopes N, Gregg D, Vasudevan S, Hassanain H, Goldschmidt-Clermont P, Kovacic H
Molecular and cellular biology 2003 Aug;23(15):5401-8
Molecular and cellular biology 2003 Aug;23(15):5401-8
Thrombospondin 2 regulates cell proliferation induced by Rac1 redox-dependent signaling.
Lopes N, Gregg D, Vasudevan S, Hassanain H, Goldschmidt-Clermont P, Kovacic H
Molecular and cellular biology 2003 Aug;23(15):5401-8
Molecular and cellular biology 2003 Aug;23(15):5401-8
Thrombospondin-1 promotes fibroblast-mediated collagen gel contraction caused by activation of latent transforming growth factor beta-1.
Sakai K, Sumi Y, Muramatsu H, Hata K, Muramatsu T, Ueda M
Journal of dermatological science 2003 Apr;31(2):99-109
Journal of dermatological science 2003 Apr;31(2):99-109
Adhesion of normal erythrocytes at depressed venous shear rates to activated neutrophils, activated platelets, and fibrin polymerized from plasma.
Goel MS, Diamond SL
Blood 2002 Nov 15;100(10):3797-803
Blood 2002 Nov 15;100(10):3797-803
Id1 regulates angiogenesis through transcriptional repression of thrombospondin-1.
Volpert OV, Pili R, Sikder HA, Nelius T, Zaichuk T, Morris C, Shiflett CB, Devlin MK, Conant K, Alani RM
Cancer cell 2002 Dec;2(6):473-83
Cancer cell 2002 Dec;2(6):473-83
Thrombospondin-1-mediated metastasis suppression by the primary tumor in human melanoma xenografts.
Rofstad EK, Graff BA
The Journal of investigative dermatology 2001 Nov;117(5):1042-9
The Journal of investigative dermatology 2001 Nov;117(5):1042-9
Mechanism of interaction of thrombospondin with human endothelium and inhibition of sickle erythrocyte adhesion to human endothelial cells by heparin.
Gupta K, Gupta P, Solovey A, Hebbel RP
Biochimica et biophysica acta 1999 Jan 6;1453(1):63-73
Biochimica et biophysica acta 1999 Jan 6;1453(1):63-73
Mechanism of interaction of thrombospondin with human endothelium and inhibition of sickle erythrocyte adhesion to human endothelial cells by heparin.
Gupta K, Gupta P, Solovey A, Hebbel RP
Biochimica et biophysica acta 1999 Jan 6;1453(1):63-73
Biochimica et biophysica acta 1999 Jan 6;1453(1):63-73
Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?
Shi SR, Cote RJ, Hawes D, Thu S, Shi Y, Young LL, Taylor CR
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999 Apr;47(4):463-70
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999 Apr;47(4):463-70
Calcium-induced modification of protein conformation demonstrated by immunohistochemistry: What is the signal?
Shi SR, Cote RJ, Hawes D, Thu S, Shi Y, Young LL, Taylor CR
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999 Apr;47(4):463-70
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 1999 Apr;47(4):463-70
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Thrombospondin using Thrombospondin Monoclonal Antibody (Product # MA5-13377) on HUVEC Cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Thrombospondin 1 was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Thrombospondin 1 (A4.1) Mouse Monoclonal Antibody (Product # MA5-13377) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L)/IgM (L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
