PAB21260

antibody from Abnova Corporation

Targeting: RRP1B KIAA0179, Nnp1, PPP1R136, RRP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21260

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21260, RRID:AB_10963902

Product name

RRP1B polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant RRP1B.

Antigen sequence

VLESEAGQPQALGSSGTCSSLKKQKLRAESDFVKF
DTPFLPKPLFFRRAKSSTATHPPGPAVQLNKTPSS
SKKVTFGLNRNMTAEFKKTDKSILVSPTGPSRVAF
DPEQKPLHGVLKTPTSSPASSPLVAKKPLTT


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with RRP1B polyclonal antibody (Cat # PAB21260) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with RRP1B polyclonal antibody (Cat # PAB21260) at 1-4 ug/mL dilution shows positivity in nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with RRP1B polyclonal antibody (Cat # PAB21260) shows strong nuclear positivity in cells in seminiferus ducts at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)