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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
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- Product number
- MA5-15700 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KLHL1 Monoclonal Antibody (3A8)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15700 targets KLHL1 in IF and WB applications and shows reactivity with Human, Mouse and Rat samples.
- Antibody clone number
- 3A8
- Concentration
- Conc. Not Determined
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KLHL1 was performed by loading 75 µg or 100 µg mouse (Ms) and rat (Rt) whole brain lysates per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked for 1 hour with TBST + 5% milk. The membrane was probed with a KLHL1 monoclonal antibody (Product # MA5-15700) at a dilution of 1:10,000 overnight at 16C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 31430) at a dilution of 1:2500 for 1 hour at room temperature. Detection was performed using SuperSignal West Femto (Product # 34095). Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KLHL1 was performed by loading 100 µg of rat hippocampal neuronal culture lysate per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane and blocked for 1 hour with TBST + 5% milk. The membrane was probed with a KLHL1 monoclonal antibody (Product # MA5-15700) at a dilution of 1:10,000 overnight at 16C, washed in TBST, and probed with an HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 31430) at a dilution of 1:2500 for 1 hour at room temperature. Detection was performed using SuperSignal West Femto (Product # 34095). Data courtesy of the Innovators Program.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KLHL1 using a KLHL1 monoclonal antibody (Product # MA5-15700) against a human KLHL1 (AA: 202-506) recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KLHL1 using a KLHL1 monoclonal antibody (Product # MA5-15700) against a human KLHL1 (AA: 202-506) recombinant protein.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using KLHL1 monoclonal antibody (Product # MA5-15700) (Green). Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using KLHL1 monoclonal antibody (Product # MA5-15700) (Green). Red: actin filaments have been labeled with phalloidin.
