AM00053PU-N

antibody from Acris Antibodies GmbH

Targeting: ERBB2 CD340, HER-2, HER2, NEU, NGL

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM00053PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00053PU-N, RRID:AB_977173

Product name

anti CD340 / ERBB2 / HER2 pTyr1248

Antibody type

Monoclonal

Antigen

Phosphopeptide conjugated to hemocyanin

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

6G7

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

erbB2 activation Serum starved OVCAR-5 cells were incubated with 10 ng/ml EGF for the indicated times. Whole cell lysates were prepared with lysis buffer V19 and separated by SDS-PAGE (ca 20.000 cells/lane). The immunoblot was probed with mab erbB2 - 6G7 (0.5 µg/ ml) for 1h at RTand developed by ECL (exp. time: 30 sec). lane 1: control; lane 2: 5 min EGF; lane 3: 15 min EGF; lane 4: 30 min EGF; lane 5: 1h EGF;lane 6; 2h EGF; lane 7: 4h EGF; lane 8: 8h EGF