Explore
Validate
Learn
Western blot
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-16950 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRYAB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This Crystallin AB antibody is useful for Western blot analysis, where a band is seen at ~20 kDa. There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex. CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes. Suggested positive control: human and mouse skeletal muscle lysate, human skeletal muscle protein, antigen standard for HSPB1 (transient overexpression lysate), mouse skeletal muscle protein. Predicted to react with rat, sheep, rabbit, monkey, guinea pig, chicken, and pig based on 100% sequence homology. Immunogen sequence has 81% homology with Zebrafish.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μL
- Concentration
- 1.08 mg/mL
- Storage
- 4°C, do not freeze
Submitted references Proteasome stress in skeletal muscle mounts a long-range protective response that delays retinal and brain aging.
WWTR1(TAZ)-CAMTA1 reprograms endothelial cells to drive epithelioid hemangioendothelioma.
Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease.
Rai M, Coleman Z, Curley M, Nityanandam A, Platt A, Robles-Murguia M, Jiao J, Finkelstein D, Wang YD, Xu B, Fan Y, Demontis F
Cell metabolism 2021 Jun 1;33(6):1137-1154.e9
Cell metabolism 2021 Jun 1;33(6):1137-1154.e9
WWTR1(TAZ)-CAMTA1 reprograms endothelial cells to drive epithelioid hemangioendothelioma.
Driskill JH, Zheng Y, Wu BK, Wang L, Cai J, Rakheja D, Dellinger M, Pan D
Genes & development 2021 Apr 1;35(7-8):495-511
Genes & development 2021 Apr 1;35(7-8):495-511
Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease.
Heaven MR, Flint D, Randall SM, Sosunov AA, Wilson L, Barnes S, Goldman JE, Muddiman DC, Brenner M
Journal of proteome research 2016 Jul 1;15(7):2265-82
Journal of proteome research 2016 Jul 1;15(7):2265-82
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4. TAZ-CAMTA1 drives a proliferative endothelial cell population with a YAP/TAZ target gene signature. ( A ) Schematic for isolation of lung ECs for scRNA-seq. ( B ) t-SNE plot of clusters of cells observed from scRNA-seq. ( C ) t-SNE plots showing expression of various marker genes. Red arrows point to cluster showing positive expression. ( D ) Volcano plot of 806 differentially expressed genes in TAZ-CAMTA1-induced proliferative subpopulation. Wilcoxon rank sum test, adjusted P -value < 0.05. Genes in red have a log(FC) > 1 and genes in blue have a log(FC) < 1. Some pertinent genes and their corresponding points are marked. ( E ) t-SNE plots of markers from proliferative subpopulation. ( F ) Immunostaining validation of differentially expressed genes identified from scRNA-seq analysis. White arrows point to positive staining of Pdgfa, Cryab, or S100a6 in P40 TAZ-CAMTA1 iEC but not Ctrl lung vessels. Scale bars, 25 um. ( G ) GSEA plot showing normalized enrichment score (NES) of YAP-conserved signature in the proliferative subpopulation, NES = 1.8. P < 0.01, FDR = 0.1. ( H ) t-SNE plots of canonical YAP/TAZ target genes. Red arrows point to the proliferative cluster showing high expression of these genes.
