PA1-16950

antibody from Invitrogen Antibodies

Targeting: CRYAB CRYA2, HSPB5

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-16950

Provider

Invitrogen Antibodies

Product name

CRYAB Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This Crystallin AB antibody is useful for Western blot analysis, where a band is seen at ~20 kDa. There is also a band of greater intensity at ~40 kDa that may be an aggregated heteromeric complex. CryAB aggregates with homologous proteins, including CryAA and the small heat shock protein HSP28, to form large heteromeric complexes.Suggested positive control: human and mouse skeletal muscle lysate, human skeletal muscle protein, antigen standard for HSPB1 (transient overexpression lysate), mouse skeletal muscle protein.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.08 mg/mL

Storage

4° C, do not freeze

References

Submitted references

Proteasome stress in skeletal muscle mounts a long-range protective response that delays retinal and brain aging.

Rai M, Coleman Z, Curley M, Nityanandam A, Platt A, Robles-Murguia M, Jiao J, Finkelstein D, Wang YD, Xu B, Fan Y, Demontis F
Cell metabolism 2021 Jun 1;33(6):1137-1154.e9

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WWTR1(TAZ)-CAMTA1 reprograms endothelial cells to drive epithelioid hemangioendothelioma.

Driskill JH, Zheng Y, Wu BK, Wang L, Cai J, Rakheja D, Dellinger M, Pan D
Genes & development 2021 Apr 1;35(7-8):495-511

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Composition of Rosenthal Fibers, the Protein Aggregate Hallmark of Alexander Disease.

Heaven MR, Flint D, Randall SM, Sosunov AA, Wilson L, Barnes S, Goldman JE, Muddiman DC, Brenner M
Journal of proteome research 2016 Jul 1;15(7):2265-82

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CRYAB in human skeletal muscle, mouse skeletal muscle. Samples were incubated in CRYAB polyclonal antibody (Product # PA1-16950 using a dilution of 0.5 µg/mL. Lane 1: Human skeletal muscle Lane 2: Mouse skeletal muscle.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-CRYAB Polyclonal Antibody (Product # PA1-16950) and a 20 kDa band corresponding to CRYAB was observed only in Mouse Eye and Rat Eye and not in any other tissues. Tissue extracts (30 µg lysate) of Mouse Eye (Lane 1), Rat Eye (Lane 2), Mouse Heart (Lane 3), Mouse Skeletal Muscle (Lane 4), Mouse Lung (Lane 5) and Mouse Liver (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/ml) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4. TAZ-CAMTA1 drives a proliferative endothelial cell population with a YAP/TAZ target gene signature. ( A ) Schematic for isolation of lung ECs for scRNA-seq. ( B ) t-SNE plot of clusters of cells observed from scRNA-seq. ( C ) t-SNE plots showing expression of various marker genes. Red arrows point to cluster showing positive expression. ( D ) Volcano plot of 806 differentially expressed genes in TAZ-CAMTA1-induced proliferative subpopulation. Wilcoxon rank sum test, adjusted P -value < 0.05. Genes in red have a log(FC) > 1 and genes in blue have a log(FC) < 1. Some pertinent genes and their corresponding points are marked. ( E ) t-SNE plots of markers from proliferative subpopulation. ( F ) Immunostaining validation of differentially expressed genes identified from scRNA-seq analysis. White arrows point to positive staining of Pdgfa, Cryab, or S100a6 in P40 TAZ-CAMTA1 iEC but not Ctrl lung vessels. Scale bars, 25 um. ( G ) GSEA plot showing normalized enrichment score (NES) of YAP-conserved signature in the proliferative subpopulation, NES = 1.8. P < 0.01, FDR = 0.1. ( H ) t-SNE plots of canonical YAP/TAZ target genes. Red arrows point to the proliferative cluster showing high expression of these genes.