PA3-514

antibody from Invitrogen Antibodies

Targeting: NR3C1 GR, GRL

Immunoprecipitation

Antibody data

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Validation data

Product number: PA3-514 - Provider product page
Provider: Invitrogen Antibodies
Product name: Glucocorticoid Receptor beta Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA3-514 detects glucocorticoid receptor (GR) beta from human cells. PA3-514 has been successfully used in Western blot, immunocytochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a ~90 kDa protein representing GR beta from HeLa cell lysate. Immunocytochemical staining of GR beta in HeLa cells with PA3-514 results in nuclear staining. PA3-514 immunizing peptide corresponds to amino acid residues 728-742 from human GR beta. This peptide (Cat. # PEP-222) is available for use in neutralization and control experiments.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 50 μL
Concentration: Conc. Not Determined
Storage: -20°C, Avoid Freeze/Thaw Cycles

NR3C1 protein structure - PA3-514 shown in red.

Supportive validation

Supportive validation

Supportive validation

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Western blot analysis of glucocorticoid receptor alpha and beta isoforms of peripheral blood mononuclear cells from septic patients . GRalpha and GRbeta cell expression were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on ICU admission (first day in the ICU) and on the day of hospital discharge, which was considered as the control situation (free of sepsis). The quantification of the western blot analysis of (A) GRalpha and (B) GRbeta is shown. The values were normalized to beta actin expression and are expressed as percentage values of the control (GR expression on the day of hospital discharge). Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). (C) Representative western blot analysis of GRalphaGRbeta and beta actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Western blot analysis of glucocorticoid receptor alpha and beta isoforms of peripheral blood mononuclear cells from septic patients on first and third day in the intensive care unit . The GRalpha and GRbeta cell expressions were evaluated by western blotting in peripheral blood mononuclear cells from septic patients on the first and third day in ICU. The quantification of the western blot analysis of (A) GRalpha and (B) GRbeta are shown. The values were normalized to beta actin expression and are expressed as percentage values of the GR expression on the first day in ICU. Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). ns = not statistically significant. (C) Representative western blot analysis of GC receptors alphabeta and beta actin are shown. GR, glucocorticoid receptor; ICU, intensive care unit.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Effect of human septic serum on glucocorticoid receptor isoform expression in different cell lines . CEM, Raji and K562 cell lines were cultured in the presence of the patients' septic serum and serum obtained from the control group at 10% final concentration in the culture medium. After 48 h in culture, the GR protein expression was analyzed by western blotting with specific antibodies for GRalpha and beta isoforms. The quantification of the western blot analysis of (A) GRalpha and (B) GRbeta are shown ( n = 9). The values were normalized to beta actin expression and are expressed as percentage values of the control (GR cell expression cultured in the presence of control serum). Data are presented as median value, 25% to 75% (box) and minimum-maximum (vertical line). Representative western blot analysis of GRalpha, beta and beta actin are shown for each cell line. CS, control serum; GR, glucocorticoid receptor; SS, septic serum.