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LearnNB600-101
antibody from Novus Biologicals
Targeting: CALR
cC1qR, CRT, FLJ26680, RO, SSA
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Protein array Blocking/Neutralizing Immunoelectron microscopyAntibody data
- Antibody Data
- Antigen structure
- References [28]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NB600-101 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-101, RRID:AB_2069792
- Product name
- Rabbit Polyclonal Calreticulin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Chemotherapy-induced ileal crypt apoptosis and the ileal microbiome shape immunosurveillance and prognosis of proximal colon cancer.
Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet - a neglected source for uEVs.
Biallelic TANGO1 mutations cause a novel syndromal disease due to hampered cellular collagen secretion.
Collectin-11 Is an Important Modulator of Retinal Pigment Epithelial Cell Phagocytosis and Cytokine Production.
Systemic chromosome instability in Shugoshin-1 mice resulted in compromised glutathione pathway, activation of Wnt signaling and defects in immune system in the lung.
Additive effect of calreticulin and translation initiation factor eIF4E on secreted protein production in the baculovirus expression system.
Α-synuclein and β-synuclein enhance secretion protein production in baculovirus expression vector system.
Enhanced protein secretion from insect cells by co-expression of the chaperone calreticulin and translation initiation factor eIF4E.
Distribution patterns of calcium-binding proteins in pancreatic tissue of non-diabetic as well as type 2 diabetic rats and in rat insulinoma beta-cells (INS-1).
Recruitment of functionally distinct membrane proteins to chromatin mediates nuclear envelope formation in vivo.
C1q enhances IFN-gamma production by antigen-specific T cells via the CD40 costimulatory pathway on dendritic cells.
Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein.
Localization of sulfonylurea receptor subunits, SUR2A and SUR2B, in rat heart.
Impaired megakaryocytopoiesis in type 2B von Willebrand disease with severe thrombocytopenia.
A splice variant of the TCR zeta mRNA lacking exon 7 leads to the down-regulation of TCR zeta, the TCR/CD3 complex, and IL-2 production in systemic lupus erythematosus T cells.
A splice variant of the TCR zeta mRNA lacking exon 7 leads to the down-regulation of TCR zeta, the TCR/CD3 complex, and IL-2 production in systemic lupus erythematosus T cells.
ATP-sensitive K+-channel subunits on the mitochondria and endoplasmic reticulum of rat cardiomyocytes.
Human FK506 binding protein 65 is associated with colorectal cancer.
Novel role of phospholipase C-delta1: regulation of liver mitochondrial Ca2+ uptake.
Transient calnexin interaction confers long-term stability on folded K+ channel protein in the ER.
Characterization of the intracellular calcium store at the base of the sperm flagellum that regulates hyperactivated motility.
C1q-calreticulin induced oxidative neurotoxicity: relevance for the neuropathogenesis of Alzheimer's disease.
C1q-calreticulin induced oxidative neurotoxicity: relevance for the neuropathogenesis of Alzheimer's disease.
Calreticulin--an endoplasmic reticulum protein with calcium-binding activity is also found in the extracellular matrix.
Calreticulin--an endoplasmic reticulum protein with calcium-binding activity is also found in the extracellular matrix.
A dominant negative mutation of neuronal connexin 36 that blocks intercellular permeability.
A dominant negative mutation of neuronal connexin 36 that blocks intercellular permeability.
Paxillin associates with poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cells.
Roberti MP, Yonekura S, Duong CPM, Picard M, Ferrere G, Tidjani Alou M, Rauber C, Iebba V, Lehmann CHK, Amon L, Dudziak D, Derosa L, Routy B, Flament C, Richard C, Daillère R, Fluckiger A, Van Seuningen I, Chamaillard M, Vincent A, Kourula S, Opolon P, Ly P, Pizzato E, Becharef S, Paillet J, Klein C, Marliot F, Pietrantonio F, Benoist S, Scoazec JY, Dartigues P, Hollebecque A, Malka D, Pagès F, Galon J, Gomperts Boneca I, Lepage P, Ryffel B, Raoult D, Eggermont A, Vanden Berghe T, Ghiringhelli F, Vandenabeele P, Kroemer G, Zitvogel L
Nature medicine 2020 Jun;26(6):919-931
Nature medicine 2020 Jun;26(6):919-931
Rigorous characterization of urinary extracellular vesicles (uEVs) in the low centrifugation pellet - a neglected source for uEVs.
Musante L, Bontha SV, La Salvia S, Fernandez-Piñeros A, Lannigan J, Le TH, Mas V, Erdbrügger U
Scientific reports 2020 Feb 28;10(1):3701
Scientific reports 2020 Feb 28;10(1):3701
Biallelic TANGO1 mutations cause a novel syndromal disease due to hampered cellular collagen secretion.
Lekszas C, Foresti O, Raote I, Liedtke D, König EM, Nanda I, Vona B, De Coster P, Cauwels R, Malhotra V, Haaf T
eLife 2020 Feb 26;9
eLife 2020 Feb 26;9
Collectin-11 Is an Important Modulator of Retinal Pigment Epithelial Cell Phagocytosis and Cytokine Production.
Dong X, Wu W, Ma L, Liu C, Bhuckory MB, Wang L, Nandrot EF, Xu H, Li K, Liu Y, Zhou W
Journal of innate immunity 2017;9(6):529-545
Journal of innate immunity 2017;9(6):529-545
Systemic chromosome instability in Shugoshin-1 mice resulted in compromised glutathione pathway, activation of Wnt signaling and defects in immune system in the lung.
Yamada HY, Kumar G, Zhang Y, Rubin E, Lightfoot S, Dai W, Rao CV
Oncogenesis 2016 Aug 15;5(8):e256
Oncogenesis 2016 Aug 15;5(8):e256
Additive effect of calreticulin and translation initiation factor eIF4E on secreted protein production in the baculovirus expression system.
Teng CY, van Oers MM, Wu TY
Applied microbiology and biotechnology 2013 Oct;97(19):8505-16
Applied microbiology and biotechnology 2013 Oct;97(19):8505-16
Α-synuclein and β-synuclein enhance secretion protein production in baculovirus expression vector system.
Teng CY, Chang SL, Tsai MF, Wu TY
Applied microbiology and biotechnology 2013 May;97(9):3875-84
Applied microbiology and biotechnology 2013 May;97(9):3875-84
Enhanced protein secretion from insect cells by co-expression of the chaperone calreticulin and translation initiation factor eIF4E.
Teng CY, Chang SL, van Oers MM, Wu TY
Molecular biotechnology 2013 May;54(1):68-78
Molecular biotechnology 2013 May;54(1):68-78
Distribution patterns of calcium-binding proteins in pancreatic tissue of non-diabetic as well as type 2 diabetic rats and in rat insulinoma beta-cells (INS-1).
Bazwinsky-Wutschke I, Wolgast S, Mühlbauer E, Peschke E
Histochemistry and cell biology 2010 Aug;134(2):115-27
Histochemistry and cell biology 2010 Aug;134(2):115-27
Recruitment of functionally distinct membrane proteins to chromatin mediates nuclear envelope formation in vivo.
Anderson DJ, Vargas JD, Hsiao JP, Hetzer MW
The Journal of cell biology 2009 Jul 27;186(2):183-91
The Journal of cell biology 2009 Jul 27;186(2):183-91
C1q enhances IFN-gamma production by antigen-specific T cells via the CD40 costimulatory pathway on dendritic cells.
Baruah P, Dumitriu IE, Malik TH, Cook HT, Dyson J, Scott D, Simpson E, Botto M
Blood 2009 Apr 9;113(15):3485-93
Blood 2009 Apr 9;113(15):3485-93
Identification of human T cell leukemia virus type 1 tax amino acid signals and cellular factors involved in secretion of the viral oncoprotein.
Jain P, Mostoller K, Flaig KE, Ahuja J, Lepoutre V, Alefantis T, Khan ZK, Wigdahl B
The Journal of biological chemistry 2007 Nov 23;282(47):34581-93
The Journal of biological chemistry 2007 Nov 23;282(47):34581-93
Localization of sulfonylurea receptor subunits, SUR2A and SUR2B, in rat heart.
Zhou M, He HJ, Suzuki R, Liu KX, Tanaka O, Sekiguchi M, Itoh H, Kawahara K, Abe H
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2007 Aug;55(8):795-804
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2007 Aug;55(8):795-804
Impaired megakaryocytopoiesis in type 2B von Willebrand disease with severe thrombocytopenia.
Nurden P, Debili N, Vainchenker W, Bobe R, Bredoux R, Corvazier E, Combrie R, Fressinaud E, Meyer D, Nurden AT, Enouf J
Blood 2006 Oct 15;108(8):2587-95
Blood 2006 Oct 15;108(8):2587-95
A splice variant of the TCR zeta mRNA lacking exon 7 leads to the down-regulation of TCR zeta, the TCR/CD3 complex, and IL-2 production in systemic lupus erythematosus T cells.
Tsuzaka K, Setoyama Y, Yoshimoto K, Shiraishi K, Suzuki K, Abe T, Takeuchi T
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3518-25
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3518-25
A splice variant of the TCR zeta mRNA lacking exon 7 leads to the down-regulation of TCR zeta, the TCR/CD3 complex, and IL-2 production in systemic lupus erythematosus T cells.
Tsuzaka K, Setoyama Y, Yoshimoto K, Shiraishi K, Suzuki K, Abe T, Takeuchi T
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3518-25
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 15;174(6):3518-25
ATP-sensitive K+-channel subunits on the mitochondria and endoplasmic reticulum of rat cardiomyocytes.
Zhou M, Tanaka O, Sekiguchi M, He HJ, Yasuoka Y, Itoh H, Kawahara K, Abe H
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2005 Dec;53(12):1491-500
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2005 Dec;53(12):1491-500
Human FK506 binding protein 65 is associated with colorectal cancer.
Olesen SH, Christensen LL, Sørensen FB, Cabezón T, Laurberg S, Orntoft TF, Birkenkamp-Demtröder K
Molecular & cellular proteomics : MCP 2005 Apr;4(4):534-44
Molecular & cellular proteomics : MCP 2005 Apr;4(4):534-44
Novel role of phospholipase C-delta1: regulation of liver mitochondrial Ca2+ uptake.
Knox CD, Belous AE, Pierce JM, Wakata A, Nicoud IB, Anderson CD, Pinson CW, Chari RS
American journal of physiology. Gastrointestinal and liver physiology 2004 Sep;287(3):G533-40
American journal of physiology. Gastrointestinal and liver physiology 2004 Sep;287(3):G533-40
Transient calnexin interaction confers long-term stability on folded K+ channel protein in the ER.
Khanna R, Lee EJ, Papazian DM
Journal of cell science 2004 Jun 15;117(Pt 14):2897-908
Journal of cell science 2004 Jun 15;117(Pt 14):2897-908
Characterization of the intracellular calcium store at the base of the sperm flagellum that regulates hyperactivated motility.
Ho HC, Suarez SS
Biology of reproduction 2003 May;68(5):1590-6
Biology of reproduction 2003 May;68(5):1590-6
C1q-calreticulin induced oxidative neurotoxicity: relevance for the neuropathogenesis of Alzheimer's disease.
Luo X, Weber GA, Zheng J, Gendelman HE, Ikezu T
Journal of neuroimmunology 2003 Feb;135(1-2):62-71
Journal of neuroimmunology 2003 Feb;135(1-2):62-71
C1q-calreticulin induced oxidative neurotoxicity: relevance for the neuropathogenesis of Alzheimer's disease.
Luo X, Weber GA, Zheng J, Gendelman HE, Ikezu T
Journal of neuroimmunology 2003 Feb;135(1-2):62-71
Journal of neuroimmunology 2003 Feb;135(1-2):62-71
Calreticulin--an endoplasmic reticulum protein with calcium-binding activity is also found in the extracellular matrix.
Somogyi E, Petersson U, Hultenby K, Wendel M
Matrix biology : journal of the International Society for Matrix Biology 2003 Apr;22(2):179-91
Matrix biology : journal of the International Society for Matrix Biology 2003 Apr;22(2):179-91
Calreticulin--an endoplasmic reticulum protein with calcium-binding activity is also found in the extracellular matrix.
Somogyi E, Petersson U, Hultenby K, Wendel M
Matrix biology : journal of the International Society for Matrix Biology 2003 Apr;22(2):179-91
Matrix biology : journal of the International Society for Matrix Biology 2003 Apr;22(2):179-91
A dominant negative mutation of neuronal connexin 36 that blocks intercellular permeability.
Placantonakis D, Cicirata F, Welsh JP
Brain research. Molecular brain research 2002 Jan 31;98(1-2):15-28
Brain research. Molecular brain research 2002 Jan 31;98(1-2):15-28
A dominant negative mutation of neuronal connexin 36 that blocks intercellular permeability.
Placantonakis D, Cicirata F, Welsh JP
Brain research. Molecular brain research 2002 Jan 31;98(1-2):15-28
Brain research. Molecular brain research 2002 Jan 31;98(1-2):15-28
Paxillin associates with poly(A)-binding protein 1 at the dense endoplasmic reticulum and the leading edge of migrating cells.
Woods AJ, Roberts MS, Choudhary J, Barry ST, Mazaki Y, Sabe H, Morley SJ, Critchley DR, Norman JC
The Journal of biological chemistry 2002 Feb 22;277(8):6428-37
The Journal of biological chemistry 2002 Feb 22;277(8):6428-37
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calreticulin Antibody [NB600-101] - Human kidney lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: Calreticulin Antibody [NB600-101] - Lane view shows a specific band for Calreticulin in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-101] - Analysis of a FFPE tissue section of human thyroid gland using 1:50 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. This representative section shows a strong positivity of Calreticulin in the follicular epithelial cells, wherein the signal was found to be very intense in the perinuclear region of the cells which correlates well with Endoplasmic reticulum localization of this protein. The para-follicular cells, endothelial cells of blood vessels (not the RBCs though) and the loose connective tissue in the section showed a weak cytoplasmic staining. Some staining was observed in the follicles/colloids also which is potentially the secreted form of Calreticulin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-101] - Analysis of a FFPE tissue section of human thyroid gland using 1:50 dilution of Calreticulin antibody. The signal was developed using HRP-DAB based detection method which followed counterstaining of the nuclei with hematoxylin. This representative section shows a strong positivity of Calreticulin in the follicular epithelial cells, wherein the signal was found to be very intense in the perinuclear region of the cells which correlates well with Endoplasmic reticulum localization of this protein. The para-follicular cells, endothelial cells and the loose connective tissue in the section showed a weak cytoplasmic staining. Some staining was observed in the follicles/colloids also which is potentially the secreted form of Calreticulin.
