NB600-103

antibody from Novus Biologicals

Targeting: CALR cC1qR, CRT, FLJ26680, RO, SSA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB600-103

Provider

Novus Biologicals

Proper citation

Novus Cat#NB600-103, RRID:AB_10001206

Product name

Rabbit Polyclonal Calreticulin Antibody

Antibody type

Polyclonal

Description

Unpurified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Multi-Study Proteomic and Bioinformatic Identification of Molecular Overlap between Amyotrophic Lateral Sclerosis (ALS) and Spinal Muscular Atrophy (SMA).

Šoltić D, Bowerman M, Stock J, Shorrock HK, Gillingwater TH, Fuller HR
Brain sciences 2018 Dec 4;8(12)

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Endoplasmic reticulum pathology and stress response in neurons precede programmed necrosis after neonatal hypoxia-ischemia.

Chavez-Valdez R, Flock DL, Martin LJ, Northington FJ
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience 2016 Feb;48:58-70

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Magnetic fractionation and proteomic dissection of cellular organelles occupied by the late replication complexes of Semliki Forest virus.

Varjak M, Saul S, Arike L, Lulla A, Peil L, Merits A
Journal of virology 2013 Sep;87(18):10295-312

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Differential inhibition of rat and human Na+-dependent taurocholate cotransporting polypeptide (NTCP/SLC10A1)by bosentan: a mechanism for species differences in hepatotoxicity.

Leslie EM, Watkins PB, Kim RB, Brouwer KL
The Journal of pharmacology and experimental therapeutics 2007 Jun;321(3):1170-8

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Impaired megakaryocytopoiesis in type 2B von Willebrand disease with severe thrombocytopenia.

Nurden P, Debili N, Vainchenker W, Bobe R, Bredoux R, Corvazier E, Combrie R, Fressinaud E, Meyer D, Nurden AT, Enouf J
Blood 2006 Oct 15;108(8):2587-95

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Calreticulin Antibody [NB600-103] - Analysis of HeLa and C6 cell lysate using calreticulin antibody at 1:1000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Simple Western: Calreticulin Antibody [NB600-103] - Lane view shows a specific band for Calreticulin in 0.2 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Calreticulin Antibody [NB600-103] - Detection of calreticulin in human kidney lysate using NB600-103 at 1:1,000. ECL exposure, 10 seconds.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Calreticulin Antibody [NB600-103] - Analysis of FFPE human prostate cancer using Calreticulin antibody at 1:20 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in glands was observed. Staining was performed by Histowiz.