AF2270

antibody from Novus Biologicals

Targeting: IL17RC IL17-RL

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2270

Provider

Novus Biologicals

Product name

Goat Polyclonal IL-17RC Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse IL-17 RC in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human IL-17 RC is observed and less than 5% cross-reactivity with recombinant mouse (rm) IL-17 RD and rmIL-17B R is observed.

Reactivity

Mouse

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Respiratory syncytial virus infection of newborn CX3CR1-deficient mice induces a pathogenic pulmonary innate immune response.

Das S, Raundhal M, Chen J, Oriss TB, Huff R, Williams JV, Ray A, Ray P
JCI insight 2017 Sep 7;2(17)

---

Th17 cells mediate clade-specific, serotype-independent mucosal immunity.

Chen K, McAleer JP, Lin Y, Paterson DL, Zheng M, Alcorn JF, Weaver CT, Kolls JK
Immunity 2011 Dec 23;35(6):997-1009

---

TLR2 deficiency leads to increased Th17 infiltrates in experimental brain abscesses.

Nichols JR, Aldrich AL, Mariani MM, Vidlak D, Esen N, Kielian T
Journal of immunology (Baltimore, Md. : 1950) 2009 Jun 1;182(11):7119-30

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of IL-17 RC in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse IL-17 RC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2270, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

IL-6 Secretion Induced by IL-17F and Neutralization by Mouse IL-17 RC Antibody. Recombinant Human IL-17F (Catalog # 1335-INS) stimulates IL-6 secretion in the NIH-3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line), as measured by the Mouse IL-6 Quantikine ELISA Kit (Catalog # M6000B). IL-6 secre-tion elicited by Recombinant Human IL-17F (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-17 RC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2270). The ND₅₀ is typically 0.6-3.6 µg/mL.