AF-295-NA

antibody from Novus Biologicals

Targeting: OSM MGC20461

Western blot (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF-295-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal Oncostatin M/OSM Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human OSM in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Specific association of increased vascular endothelial growth factor expression and its receptors with macrophage differentiation of HL-60 leukemia cells.

Ohsaka A, Hirota-Komatsu S, Shibata M, Ezaki J, Shinohara F, Yoshida T
Biochemical and biophysical research communications 2008 Apr 11;368(3):543-9

---

The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.

He W, Gong K, Smith DK, Ip NY
FEBS letters 2005 Aug 15;579(20):4317-23

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human Oncostatin M/OSM Antibody. Recombinant Human Oncostatin M/OSM (Catalog # 295-OM) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Oncostatin M/OSM (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Oncostatin M/OSM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-295-NA). The ND₅₀ is typically 0.02-0.04 µg/mL.