653604

antibody from BioLegend

Targeting: RUNX3 AML2, CBFA3, PEBP2A3

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

653604

Provider

BioLegend

Proper citation

BioLegend Cat#653604, RRID:AB_2687118

Product name

Go-ChIP-Grade™ Purified anti-RUNX3

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

9F4A17

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Other assay

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Chromatin Immunoprecipitation (ChIP) was performed using commercial Protein-G coated 96 well high-throughput ChIP assay kit by loading 3µg of cross-linked chromatin samples from Jurkat cells with either A) 1:50 dilution of Go-ChIP-Grade™ Purified anti-Runx3 Antibody (Clone 9F4A17), B) equal amount of Purified Mouse IgG2a, κ Isotype Control Antibody, or C) competitor’s ChIP-grade Purified anti-Runx3 Antibody and D) equal amount of matched Isotype Control Antibody as recommended by the manufacturer. The enriched DNA was purified and quantified by real-time qPCR using primers targeting human PR3 gene region. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the 5% of total amount of input chromatin.

Conjugate

Unconjugated