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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00000864-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000864-B01P, RRID:AB_10731087
- Product name
- RUNX3 purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human RUNX3 protein.
- Antigen sequence
MASNSIFDSFPTYSPTFIRDPSTSRRFTPPSPAFP
CGGGGGKMGENSGALSAQAAVGPGGRARPEVRSMV
DVLADHAGELVRTDSPNFLCSVLPSHWRCNKTLPV
AFKVVALGDVPDGTVVTVMAGNDENYSAELRNASA
VMKNQVARFNDLRFVGRSGRGKSFTLTITVFTNPT
QVATYHRAIKVTVDGPREPRRHRQKLEDQTKPFPD
RFGDLERLRMRVTPSTPSPRGSLSTTSHFSSQPQT
PIQGTSELNPFSDPRQFDRSFPTLPTLTESRFPDP
RMHYPGAMSAAFPYSATPSGTSISSLSVAGMPATS
RFHHTYLPPPYPGAPQNQSGPFQANPSPYHLYYGT
SSGSYQFSMVAGSSSGGDRSPTRMLASCTSSAASV
AAGNLMNPSLGGQSDGVEADGSHSNSPTALSTPGR
MDEAVWRPY- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Inactivation of RUNX3 predicts poor prognosis in esophageal squamous cell carcinoma after Ivor-Lewis esophagectomy.
Shi M, Wang Z, Liu XY, Chen D
Medical oncology (Northwood, London, England) 2014 Dec;31(12):309
Medical oncology (Northwood, London, England) 2014 Dec;31(12):309
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of RUNX3 expression in transfected 293T cell line (H00000864-T01) by RUNX3 MaxPab polyclonal antibody.Lane 1: RUNX3 transfected lysate(47.19 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- RUNX3 MaxPab polyclonal antibody. Western Blot analysis of RUNX3 expression in human pancreas.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to RUNX3 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of purified MaxPab antibody to RUNX3 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
