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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- 10-4053-25 - Provider product page
- Provider
- ABEOMICS Inc.
- Product name
- Anti-RunX3 Antibody
- Antibody type
- Monoclonal
- Description
- Runt-related transcription factor 3 (Runx3) forms a heterodimer of alpha and beta subunit and binds to the core of the promoter to regulate the transcription. It plays an important role in tumor suppressor and the gene is frequently deleted or transcriptionally silenced during tumor development. It has multiple isoforms coded by multiple transcript variances.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
A partial length recombinant Runx3
protein (amino acids 100-320) was u
sed as the immunogen for this antib
ody.- Isotype
- IgG
- Antibody clone number
- ABM28D1
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeat freez thawing
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Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Western blot analysis of RunX3. Anti- RunX3 antibody (Clone: ABM28D1) was used at 2 µg/ml on (1) HL-60 and (2) Jurkat lysates.
- Protocol
- Protocol
Supportive validation
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flowcytometry analysis of RunX3 on Raji cells using 0.5 µg/10^6 Cells of Anti-RunX3 (Clone: ABM28D1). Green represent isotype control and red represent Anti RunX3 antibody (Abeomics 10-4053). Goat anti mouse PE conjugated was used as the secondary antibody.
- Protocol
- Protocol
- Submitted by
- ABEOMICS Inc. (provider)
- Main image
- Experimental details
- Intracellular flowcytometry analysis of RunX3 on Ramos cells using 0.5 µg/10^6 Cells of Anti-RunX3 (Clone: ABM28D1). Green represent isotype control and red represent Anti RunX3 antibody (Abeomics 10-4053). Goat anti mouse PE conjugated was used as the secondary antibody.
- Protocol
- Protocol
