Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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Validation data
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- Product number
- ALX-803-320-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-803-320-C100, RRID:AB_2052516
- Product name
- RUNX3 monoclonal antibody (R3-1E10)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- R3-1E10
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.
Submitted references The RUNX3 tumor suppressor upregulates Bim in gastric epithelial cells undergoing transforming growth factor beta-induced apoptosis.
Yano T, Ito K, Fukamachi H, Chi XZ, Wee HJ, Inoue K, Ida H, Bouillet P, Strasser A, Bae SC, Ito Y
Molecular and cellular biology 2006 Jun;26(12):4474-88
Molecular and cellular biology 2006 Jun;26(12):4474-88
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis using MAb to RUNX3 (R3-1E10) (Prod. No. ALX-803-320). Method: 0.2µg/ml of MAb to RUNX3 (R3-1E10) were applied to the membrane on which recombinant human (lanes 1-3) or mouse (lanes 4-6) RUNX1, RUNX2 or RUNX3 expressed in COS7 cells were loaded (see [1]).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunohistochemistry using MAb to RUNX3 (R3-1E10) (Prod. No. ALX-803-320). Method: 2µg/ml of MAb to RUNX3 (R3-1E10) were applied to normal human gastric samples (paraffin sections) which were retrieved by autoclave at 105¡C for 15 min. in Dako solution (see [1]).