ALX-803-318-C100

antibody from Enzo Life Sciences

Targeting: RUNX3 AML2, CBFA3, PEBP2A3

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

ALX-803-318-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-803-318-C100, RRID:AB_2052515

Product name

RUNX3 (human) monoclonal antibody (R3-6E9)

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

R3-6E9

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.

References

Submitted references

Targets of genome copy number reduction in primary breast cancers identified by integrative genomics.

Chen W, Salto-Tellez M, Palanisamy N, Ganesan K, Hou Q, Tan LK, Sii LH, Ito K, Tan B, Wu J, Tay A, Tan KC, Ang E, Tan BK, Tan PH, Ito Y, Tan P
Genes, chromosomes & cancer 2007 Mar;46(3):288-301

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RUNX3 is frequently inactivated by dual mechanisms of protein mislocalization and promoter hypermethylation in breast cancer.

Lau QC, Raja E, Salto-Tellez M, Liu Q, Ito K, Inoue M, Putti TC, Loh M, Ko TK, Huang C, Bhalla KN, Zhu T, Ito Y, Sukumar S
Cancer research 2006 Jul 1;66(13):6512-20

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RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization.

Ito K, Liu Q, Salto-Tellez M, Yano T, Tada K, Ida H, Huang C, Shah N, Inoue M, Rajnakova A, Hiong KC, Peh BK, Han HC, Ito T, Teh M, Yeoh KG, Ito Y
Cancer research 2005 Sep 1;65(17):7743-50

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis using MAb to RUNX3 (human) (R3-6E9) (Prod. No. ALX-803-318). Method: 0.2µg/ml of MAb to RUNX3 (human) (R3-6E9) were applied to the membrane on which recombinant human (lanes 1-3) or mouse (lanes 4-6) RUNX1, RUNX2Êor RUNX3 expressed in COS7 cells were loaded (see [1]).

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunohistochemistry using MAb to RUNX3 (human) (R3-6E9) (Prod. No. ALX-803-318). Method: 2µg/ml of MAb to RUNX3 (human) (R3-6E9) were appliedÊto normal human gastic samples (paraffin sections) which were retrieved by autoclave at 105¡C for 15 min. in Dako solution (see [1]).