AF361

antibody from Novus Biologicals

Targeting: CCL19 CKb11, ELC, exodus-3, MIP-3b, SCYA19

Western blot (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AF361

Provider

Novus Biologicals

Product name

Goat Polyclonal CCL19/MIP-3 beta Antibody

Antibody type

Polyclonal

References

Submitted references

Age-Related Changes to Human Tear Composition.

Micera A, Di Zazzo A, Esposito G, Longo R, Foulsham W, Sacco R, Sgrulletta R, Bonini S
Investigative ophthalmology & visual science 2018 Apr 1;59(5):2024-2031

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Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus.

Connelly KL, Kandane-Rathnayake R, Hoi A, Nikpour M, Morand EF
Scientific reports 2016 Jul 25;6:29909

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Expression of podoplanin and other lymphatic markers in the human anterior eye segment.

Birke K, Lütjen-Drecoll E, Kerjaschki D, Birke MT
Investigative ophthalmology & visual science 2010 Jan;51(1):344-54

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CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.

Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S
European journal of immunology 2008 Nov;38(11):3050-9

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Autologous chemotaxis as a mechanism of tumor cell homing to lymphatics via interstitial flow and autocrine CCR7 signaling.

Shields JD, Fleury ME, Yong C, Tomei AA, Randolph GJ, Swartz MA
Cancer cell 2007 Jun;11(6):526-38

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human CCL19/MIP-3 beta by Western Blot. Western blot shows conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 μg/mL LPS and 40 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL19/MIP-3 beta at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human CCL19/MIP-3 beta by Simple Western^TM. Simple Western lane view shows conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 μg/mL LPS and 40 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 24 hours. A specific band was detected for CCL19/MIP-3 beta at approximately 8 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Chemotaxis Induced by CCL19/MIP-3 beta and Neutrali-zation by Human CCL19/MIP-3 beta Antibody. Re-combinant Human CCL19/MIP-3 beta (Catalog # 361-MI) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361). The ND₅₀ is typically 2-10 µg/mL.