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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB361-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL19/MIP-3 beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CCL19/MIP-3 beta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 15, 16, 17, 18, 20, 21, 22, 23, 24, 25, recombinant mouse CCL2, 3, 4, 5, 6, 7, 9, 11, 12, 21, 22, or 25 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q99731.1- Isotype
- IgG
- Antibody clone number
- 54909
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references An initial investigation into endothelial CC chemokine expression in the human rheumatoid synovium.
Induction of immunoregulatory CD271+ cells by metastatic tumor cells that express human endogenous retrovirus H.
Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
CCL21 overexpressed on lymphatic vessels drives thymic hyperplasia in myasthenia.
Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
Rump L, Mattey DL, Kehoe O, Middleton J
Cytokine 2017 Sep;97:133-140
Cytokine 2017 Sep;97:133-140
Induction of immunoregulatory CD271+ cells by metastatic tumor cells that express human endogenous retrovirus H.
Kudo-Saito C, Yura M, Yamamoto R, Kawakami Y
Cancer research 2014 Mar 1;74(5):1361-70
Cancer research 2014 Mar 1;74(5):1361-70
Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
Pinto S, Martínez-Romero A, O'Connor JE, Gil-Benso R, San-Miguel T, Terrádez L, Monteagudo C, Callaghan RC
BMC cancer 2014 Feb 22;14:118
BMC cancer 2014 Feb 22;14:118
CCL21 overexpressed on lymphatic vessels drives thymic hyperplasia in myasthenia.
Berrih-Aknin S, Ruhlmann N, Bismuth J, Cizeron-Clairac G, Zelman E, Shachar I, Dartevelle P, de Rosbo NK, Le Panse R
Annals of neurology 2009 Oct;66(4):521-31
Annals of neurology 2009 Oct;66(4):521-31
Cutting edge: nonproliferating mature immune cells form a novel type of organized lymphoid structure in idiopathic pulmonary fibrosis.
Marchal-Sommé J, Uzunhan Y, Marchand-Adam S, Valeyre D, Soumelis V, Crestani B, Soler P
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):5735-9
Journal of immunology (Baltimore, Md. : 1950) 2006 May 15;176(10):5735-9
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL19/MIP-3 beta in Human PBMCs. CCL19/MIP-3 beta was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human CCL19/MIP-3 beta Monoclonal Anti-body (Catalog # MAB361) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL19/MIP-3 beta in Human Tonsil. CCL19/MIP-3 beta was detected in immersion fixed paraffin-embedded sections of human tonsil using 25 µg/mL Mouse Anti-Human CCL19/ MIP-3 beta Monoclonal Antibody (Catalog # MAB361) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CCL19/MIP-3 beta in Human Dendritic Cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Mouse Anti-Human CCL19/MIP-3 beta Monoclonal Antibody (Catalog # MAB361, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).
