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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Western blot [1]
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- Product number
- AF3305 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Coagulation Factor VII Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Coagulation Factor VII in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Factor VII, rhFactor X, and rhFactor Xa is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P70375- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Chimeric antigen receptor-modified T Cells inhibit the growth and metastases of established tissue factor-positive tumors in NOG mice.
Targeting tissue factor on tumour cells and angiogenic vascular endothelial cells by factor VII-targeted verteporfin photodynamic therapy for breast cancer in vitro and in vivo in mice.
Zhang Q, Wang H, Li H, Xu J, Tian K, Yang J, Lu Z, Zheng J
Oncotarget 2017 Feb 7;8(6):9488-9499
Oncotarget 2017 Feb 7;8(6):9488-9499
Targeting tissue factor on tumour cells and angiogenic vascular endothelial cells by factor VII-targeted verteporfin photodynamic therapy for breast cancer in vitro and in vivo in mice.
Hu Z, Rao B, Chen S, Duanmu J
BMC cancer 2010 May 26;10:235
BMC cancer 2010 May 26;10:235
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Mouse Coagulation Factor VII by Western Blot. Western blot shows lysate of mouse placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Coagulation Factor VII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3305) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Coagulation Factor VII at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
