BS4459

antibody from Bioworld Technology, Inc

Targeting: NR3C1 GR, GRL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

BS4459

Provider

Bioworld Technology, Inc

Product name

GR (phospho S211) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic phosphopeptide derived from human GR around the phosphorylation site of Serine 211.

Description

The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GR Ï«. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis, cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the non-ligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Bioworld Technology, Inc (provider)

Main image

Experimental details

Western blot (WB) analysis of p-GR (S211) pAb at 1:500 dilutionLane1:Hela cell lysate treated with PMA(100nM,15mins)Lane2:Raw264.7 cell lysate treated with PMA(100nM,15mins)Lane3:PC12 cell lysate treated with PMA(100nM,15mins)