NB300-731
antibody from Novus Biologicals
Targeting: NR3C1
GR, GRL
Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [5]
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Validation data
Reference
Comment
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- Product number
- NB300-731 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-731, RRID:AB_2298869
- Product name
- Mouse Monoclonal GR/NR3C1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
- Reactivity
- Human, Mouse, Rat, Guinea Pig, Rabbit, Sheep, Yeast
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references In utero Exposure to Maternal Chronic Inflammation Transfers a Pro-Inflammatory Profile to Generation F2 via Sex-Specific Mechanisms.
Chronic Gestational Inflammation: Transfer of Maternal Adaptation over Two Generations of Progeny.
Single-Cell Resolution and Quantitation of Targeted Glucocorticoid Delivery in the Thymus.
Inhibitory Effects of Prunella vulgaris L. Extract on 11β-HSD1 in Human Skin Cells.
Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels.
Functionally distinct patterns of nucleosome remodeling at enhancers in glucocorticoid-treated acute lymphoblastic leukemia.
Behavioural consequences of p-glycoprotein deficiency in mice, with special focus on stress-related mechanisms.
Steroid hormones induce bcl-X gene expression through direct activation of distal promoter P4.
Evidence for glucocorticoid receptor transport on microtubules by dynein.
CBP recruitment and histone acetylation in differential gene induction by glucocorticoids and progestins.
All of the factors required for assembly of the glucocorticoid receptor into a functional heterocomplex with heat shock protein 90 are preassociated in a self-sufficient protein folding structure, a "foldosome".
Adams RCM, Smith C
Frontiers in immunology 2020;11:48
Frontiers in immunology 2020;11:48
Chronic Gestational Inflammation: Transfer of Maternal Adaptation over Two Generations of Progeny.
Adams RCM, Smith C
Mediators of inflammation 2019;2019:9160941
Mediators of inflammation 2019;2019:9160941
Single-Cell Resolution and Quantitation of Targeted Glucocorticoid Delivery in the Thymus.
Taves MD, Mittelstadt PR, Presman DM, Hager GL, Ashwell JD
Cell reports 2019 Mar 26;26(13):3629-3642.e4
Cell reports 2019 Mar 26;26(13):3629-3642.e4
Inhibitory Effects of Prunella vulgaris L. Extract on 11β-HSD1 in Human Skin Cells.
Roh KB, Park D, Jung E
Evidence-based complementary and alternative medicine : eCAM 2018;2018:1762478
Evidence-based complementary and alternative medicine : eCAM 2018;2018:1762478
Targeting CAG repeat RNAs reduces Huntington's disease phenotype independently of huntingtin levels.
Rué L, Bañez-Coronel M, Creus-Muncunill J, Giralt A, Alcalá-Vida R, Mentxaka G, Kagerbauer B, Zomeño-Abellán MT, Aranda Z, Venturi V, Pérez-Navarro E, Estivill X, Martí E
The Journal of clinical investigation 2016 Nov 1;126(11):4319-4330
The Journal of clinical investigation 2016 Nov 1;126(11):4319-4330
Functionally distinct patterns of nucleosome remodeling at enhancers in glucocorticoid-treated acute lymphoblastic leukemia.
Wu JN, Pinello L, Yissachar E, Wischhusen JW, Yuan GC, Roberts CWM
Epigenetics & chromatin 2015;8:53
Epigenetics & chromatin 2015;8:53
Behavioural consequences of p-glycoprotein deficiency in mice, with special focus on stress-related mechanisms.
Schoenfelder Y, Hiemke C, Schmitt U
Journal of neuroendocrinology 2012 May;24(5):809-17
Journal of neuroendocrinology 2012 May;24(5):809-17
Steroid hormones induce bcl-X gene expression through direct activation of distal promoter P4.
Viegas LR, Vicent GP, Barañao JL, Beato M, Pecci A
The Journal of biological chemistry 2004 Mar 12;279(11):9831-9
The Journal of biological chemistry 2004 Mar 12;279(11):9831-9
Evidence for glucocorticoid receptor transport on microtubules by dynein.
Harrell JM, Murphy PJ, Morishima Y, Chen H, Mansfield JF, Galigniana MD, Pratt WB
The Journal of biological chemistry 2004 Dec 24;279(52):54647-54
The Journal of biological chemistry 2004 Dec 24;279(52):54647-54
CBP recruitment and histone acetylation in differential gene induction by glucocorticoids and progestins.
Lambert JR, Nordeen SK
Molecular endocrinology (Baltimore, Md.) 2003 Jun;17(6):1085-94
Molecular endocrinology (Baltimore, Md.) 2003 Jun;17(6):1085-94
All of the factors required for assembly of the glucocorticoid receptor into a functional heterocomplex with heat shock protein 90 are preassociated in a self-sufficient protein folding structure, a "foldosome".
Hutchison KA, Dittmar KD, Pratt WB
The Journal of biological chemistry 1994 Nov 11;269(45):27894-9
The Journal of biological chemistry 1994 Nov 11;269(45):27894-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in NIH/3T3 cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor in HeLa cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of GR in Jurkat cells compared to an isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - An intracellular stain was performed on Jurkat cells with GR/NR3C1 (BuGR2) antibody NB300-731PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerthrin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.