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LearnAF-426-PB
antibody from R&D Systems
Targeting: TNFRSF1B
CD120b, p75, TNF-R-II, TNF-R75, TNFBR, TNFR2, TNFR80
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- AF-426-PB - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TNF RII/TNFRSF1B Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse TNF RII in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TNF RII is observed and less than 1% cross-reactivity with recombinant mouse (rm) CD27, rmGITR, rhDR6, rmCD30, rmCD40, rmEDAR, rmFas, rmOPG, rmRANK, and rmTNF RI is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P25119- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Endogenous TNFα orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation.
Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2.
Phenotypic screening reveals TNFR2 as a promising target for cancer immunotherapy.
Myosin light chain kinase expression induced via tumor necrosis factor receptor 2 signaling in the epithelial cells regulates the development of colitis-associated carcinogenesis.
Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients.
Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
Arokiasamy S, Zakian C, Dilliway J, Wang W, Nourshargh S, Voisin MB
Scientific reports 2017 Mar 13;7:44189
Scientific reports 2017 Mar 13;7:44189
Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2.
Fischer R, Marsal J, Guttà C, Eisler SA, Peters N, Bethea JR, Pfizenmaier K, Kontermann RE
Scientific reports 2017 Jul 26;7(1):6607
Scientific reports 2017 Jul 26;7(1):6607
Phenotypic screening reveals TNFR2 as a promising target for cancer immunotherapy.
Williams GS, Mistry B, Guillard S, Ulrichsen JC, Sandercock AM, Wang J, González-Muñoz A, Parmentier J, Black C, Soden J, Freeth J, Jovanović J, Leyland R, Al-Lamki RS, Leishman AJ, Rust SJ, Stewart R, Jermutus L, Bradley JR, Bedian V, Valge-Archer V, Minter R, Wilkinson RW
Oncotarget 2016 Oct 18;7(42):68278-68291
Oncotarget 2016 Oct 18;7(42):68278-68291
Myosin light chain kinase expression induced via tumor necrosis factor receptor 2 signaling in the epithelial cells regulates the development of colitis-associated carcinogenesis.
Suzuki M, Nagaishi T, Yamazaki M, Onizawa M, Watabe T, Sakamaki Y, Ichinose S, Totsuka M, Oshima S, Okamoto R, Shimonaka M, Yagita H, Nakamura T, Watanabe M
PloS one 2014;9(2):e88369
PloS one 2014;9(2):e88369
Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients.
Haga E, Endo Y, Haruta M, Koba C, Matsumura K, Takamatsu K, Ikeda T, Nishimura Y, Senju S
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):2024-33
Journal of immunology (Baltimore, Md. : 1950) 2014 Aug 15;193(4):2024-33
Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S
The Journal of experimental medicine 2012 Jun 4;209(6):1219-34
The Journal of experimental medicine 2012 Jun 4;209(6):1219-34
TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
The Journal of clinical investigation 2009 Oct;119(10):2954-64
The Journal of clinical investigation 2009 Oct;119(10):2954-64
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse TNF RII/TNFRSF1B by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 1 µg/mL LPS for 24 hours. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TNF RII/TNFRSF1B at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TNF RII/TNFRSF1B in L-929 Mouse Cell Line by Flow Cytometry. L-929 mouse fibroblast cell line was stained with Goat Anti-Mouse TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-426-PB, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).
