AF726
antibody from R&D Systems
Targeting: TNFRSF1B
CD120b, p75, TNF-R-II, TNF-R75, TNFBR, TNFR2, TNFR80
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF726 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TNF RII/TNFRSF1B Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TNF RII/TNFRSF1B in direct ELISAs and Western blots. In direct ELISAs, less than 50% cross-reactivity with recombinant mouse TNF RII is observed and less than 5% cross-reactivity with recombinant rat TNF RII is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Mesenchymal stem cells inhibit cutaneous radiation-induced fibrosis by suppressing chronic inflammation.
Horton JA, Hudak KE, Chung EJ, White AO, Scroggins BT, Burkeen JF, Citrin DE
Stem cells (Dayton, Ohio) 2013 Oct;31(10):2231-41
Stem cells (Dayton, Ohio) 2013 Oct;31(10):2231-41
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- TNF RII/TNFRSF1B Inhibition of TNF-alpha-induced Cytotoxicity and Neutralization by Human TNF RII/TNFRSF1B Antibody. Recombinant Human TNF RII/TNFRSF1B Fc Chimera (Catalog # 726-R2) inhibits Recombinant Human TNF-alpha (Catalog # 210-TA) induced cytotoxicity in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Inhibition of Recombinant Human TNF-alpha (0.25 ng/mL) activity elicited by Recombinant Human TNF RII/TNFRSF1B Fc Chimera (0.075 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TNF RII/TNFRSF1B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF726). The ND50 is typically 0.04-0.2 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).