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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00055869-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00055869-M01, RRID:AB_669382
- Product name
- Mouse Monoclonal Histone Deacetylase 8/HDAC8 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. histone deacetylase 8
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Transcription regulation mechanism of the syntaxin 1A gene via protein kinase A.
Nakayama T, Akagawa K
The Biochemical journal 2017 Jul 11;474(14):2465-2473
The Biochemical journal 2017 Jul 11;474(14):2465-2473
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01] - Analysis of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M01), clone 2F4.Lane 1: HDAC8 transfected lysate(41.8 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01] - HDAC8 monoclonal antibody (M01), clone 2F4 Analysis of HDAC8 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01] - Detection limit for recombinant GST tagged HDAC8 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (2F4) [H00055869-M01] - Analysis of monoclonal antibody to HDAC8 on HeLa cell. Antibody concentration 10 ug/ml.
