CABT-L817

antibody from Creative Diagnostics

Targeting: HDAC8 HDACL1, KDAC8, MRXS6, RPD3, WTS

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

CABT-L817

Provider

Creative Diagnostics

Product name

Rabbit Anti-Human HDAC8 monoclonal antibody, clone KK1956

Antibody type

Monoclonal

Description

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

KK1956

Vial size

100 _l

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.