600-401-421

antibody from Rockland Immunochemicals, Inc.

Targeting: PPARA hPPAR, NR1C1, PPAR

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-421

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-421, RRID:AB_2165756

Product name

Anti-PPAR alpha (N-terminal specific) (RABBIT) Antibody - 600-401-421

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

Role of Adiponectin-Notch pathway in cognitive dysfunction associated with depression and in the therapeutic effect of physical exercise.

You J, Sun L, Wang J, Sun F, Wang W, Wang D, Fan X, Liu D, Xu Z, Qiu C, Chen J, Yan H, Liu B
Aging cell 2021 Jun;20(6):e13387

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Activation of peroxisome proliferator-activated receptor α stimulates ADAM10-mediated proteolysis of APP.

Corbett GT, Gonzalez FJ, Pahan K
Proceedings of the National Academy of Sciences of the United States of America 2015 Jul 7;112(27):8445-50

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Affinity Purified Anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 µg of lysate was loaded per lane for SDS-PAGE.  Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization. Storage Conditions:   Store vial at -20° C prior to opening.  Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Expiration date is one (1) year from date of opening.

Validation comment

Western Blot