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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- ARG55240 - Provider product page
- Provider
- Arigo
- Product name
- anti-PPAR alpha antibody
- Antibody type
- Monoclonal
- Antigen
- Recombination protein of Human PPAR alpha (Swiss: Q07869).
- Description
- Purification with Protein G.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1331CT894.186.143
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 30 µg of 1) HepG2, 2) HEK293T, 3) HCT116, 4) NIH-3T3, 5) Mouse brain, 6) rat brain, and 7) Rat ovary lysate stained with ARG55240 anti-PPAR alpha antibody at 1:500 dilution.Data provided by customer.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of HeLa, Jurkat, mouse NIH/3T3 cell line (from left to right) stained with ARG55240 anti-PPAR alpha antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells stained with ARG55240 anti-PPAR alpha antibody (green) at 1:25 dilution. Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded H. skeletal muscle section stained with ARG55240 anti-PPAR alpha antibody at 1:25 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded H. kidney section stained with ARG55240 anti-PPAR alpha antibody at 1:25 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG55240 anti-PPAR alpha antibody (green) at 1:25 dilution or without primary antibodies (blue), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
