R1489P

antibody from Acris Antibodies GmbH

Targeting: PPARA hPPAR, NR1C1, PPAR

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R1489P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1489P, RRID:AB_1006252

Product name

anti PPAR-alpha (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 1 to 18 of Human PPAR alpha.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Hamster

Host

Rabbit

Vial size

0.1 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunoblotting: Affinity Purified Anti-PPAR alpha (N-terminal specific) is shown to detect a 52 kDa band corresponding to Human PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 µg of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1/500 (Lane 1) or 1/1000 (Lane 2) dilution of antibody followed by 1/2000 dilution of HRP Goat anti-Rabbit IgG for visualization.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry using PPAR antibody, showing staining of PPAR alpha in Rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurones). Top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry showing PPAR alpha antibody staining of PPAR alpha protein in Formalin/PFA-Fixed Paraffin-Embedded Mouse liver tissue section. Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20°C. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20°C. A Biotin conjugated Goat polyclonal to Rabbit IgG was used at dilution at 1/500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).